FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386655
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGA200.007362069651.73805629
TAGCGCT350.00134823739.4194729
CTAGCGC350.00134823739.4194728
GTATCAA10700.038.9646641
GGTATCA5450.036.0369221
CGCGTCT501.7471125E-434.86665751
CTCGTAT400.002548048734.6265139
CGGGTCG400.002610366834.45635617
TATCAAC12550.032.6717642
ATCTGCG652.0408977E-532.4435863
TCAACGC12900.031.785324
CAACGCA13050.031.4199735
ATCAACG13050.031.4199733
AACGCAG13350.030.9720066
ATGCCGT450.00445672430.8915344
ATAAGAC450.00464762630.6278743
TAAGACT802.7827227E-630.1493114
ACGCAGA13750.030.0710017
AGAGTAC14000.029.287911
CGCAGAG14150.029.2209368
Produced by FastQC (version 0.11.5)