FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386655
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT252.4282667E-455.81553352
TCGCTAG305.8482104E-446.70899258
TTCGCTA350.00125155640.02046257
GTATCAA11400.038.4143521
GTGGTAT2200.037.6169171
GGTATCA5050.036.1892131
CGCTAGC400.0023911635.0779559
TATCAAC13050.033.2586362
ATCAACG13100.032.8304753
TCAACGC13200.032.569124
CAACGCA13250.032.446225
AACGCAG13450.031.9637496
TAGCGTG553.312861E-431.2663575
AGGTACG450.004389011330.98830649
ACGCAGA14100.030.4902387
CGCAGAG14150.030.3825048
CAGAGTA14700.029.47970810
AGAGTAC14900.029.08400711
TTAGCGT605.5149716E-428.6608284
CGCGAGT500.00759251827.70066538
Produced by FastQC (version 0.11.5)