FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658835
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC7390.11216768993754127No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6770.102757139496232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC200.007452768351.58213813
GGTATCA7350.042.5789641
GTGTTAT602.98136E-740.1224861
GTATCAA17150.040.1057741
CGCTAAT400.002615691634.44560632
TCAACGC21350.032.374744
CAACGCA21500.032.308815
ATCAACG21750.031.779343
TATCAAC22000.031.574522
AGTGCGA553.3181446E-431.26190410
AACGCAG22200.031.1351646
ACGCAGA23750.029.1031847
CGCAGAG24350.028.3860638
AGAGTAC25000.027.51047311
CAGAGTA24950.027.42777610
GTGGTAT2850.026.5472091
GCAGAGT26600.025.8557079
AGTTTCG851.07505955E-425.43003367
TGGTATC3050.024.8045252
TACGGGT2650.024.65561315
Produced by FastQC (version 0.11.5)