FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668341
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44560.6667255188593847No Hit
GTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAA32590.48762532898625105No Hit
GTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTT30990.4636854539823234No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30970.4633862055447743No Hit
GTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCT30590.4577004852313415No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29410.4400448274159448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26380.3947086891272569No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25620.38333724850039125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24730.3700206930294565No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG22660.33904847974312513No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTC22650.3388988555243506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21550.3224401914591503No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21520.3219913188028267No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG20980.3139116109890011No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC19540.29236572348546624No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18870.2823409008275715No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT18590.2781514227018842No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGA17990.26917396957541134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17650.2640867461370767No Hit
GACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCT17440.2609446375428112No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA17250.2581017773860948No Hit
GGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGG17240.2579521531673203No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16590.2482265789469747No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGACCAAATGCG16350.24463559769638551No Hit
CATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15200.22742881253731254No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15170.2269799398809889No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15130.22638144300589072No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCT15120.22623181878711615No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15110.2260821945683416No Hit
CTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT15070.22548369769324345No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14970.22398745550549795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14950.22368820706794887No Hit
GAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14790.22129421956755607No Hit
CCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTC14770.22099497113000696No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACG14520.21725436566064332No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14500.21695511722309418No Hit
AGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT13830.20693029456519949No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13530.20244156800196308No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCC13300.19900021097014847No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13300.19900021097014847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13230.19795284143872663No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13000.19451148440691204No Hit
GTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG12850.19226712112529382No Hit
CTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAG12590.18837689143715558No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12510.1871798976869592No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12360.184935534405341No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12200.18254154690494823No Hit
GCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCC11900.1780528203417118No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11590.1734144695597008No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11500.17206785159072988No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11320.16937461565278802No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGT11300.16907536721523891No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11290.16892574299646437No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11090.16593325862097344No Hit
GTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGG11040.1651851375271007No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGG10970.16413776799567883No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10970.16413776799567883No Hit
GCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGT10960.1639881437769043No Hit
GACCAAATGCGTACGGGGCCCGTATACTTTCCATGCAGTAATAAACCCCA10860.16249190158915883No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA10650.15934979299489332No Hit
TCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACA10530.15755430236959875No Hit
CCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAG10290.15396332111900962No Hit
CTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGT10220.15291595158758778No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10220.15291595158758778No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10170.15216783049371504No Hit
AGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAG10090.15097083674351863No Hit
GTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA10060.150521964087195No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA10000.14962421877454773No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9950.14887609768067497No Hit
ACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTT9760.14603323752395858No Hit
ATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAG9700.14513549221131128No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9690.14498586799253674No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9680.1448362437737622No Hit
GATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCC9670.14468661955498763No Hit
GTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGA9610.14378887424234035No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9580.14334000158601673No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGAC9570.14319037736724216No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9490.1419933836170458No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTC9450.1413948867419476No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9450.1413948867419476No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAC9300.13915052346032938No Hit
CCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCC9250.13840240236645665No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAATACGTCTCTCCA8930.13361442736567114No Hit
CTCCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC8720.1304723187714056No Hit
GTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT8580.12837757970856195No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8530.12762945861468922No Hit
GGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGAT8530.12762945861468922No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8500.12718058595836557No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8490.127030961739591No Hit
GTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCT8320.1244873500204237No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8260.12358960470777643No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8250.12343998048900187No Hit
CTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGG8190.1225422351763546No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8160.12209336252003095No Hit
ATCTGGCACTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA7990.11954975080086362No Hit
TCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT7900.1182031328318927No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCAC7850.11745501173801996No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7810.11685651486292177No Hit
TCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG7790.11655726642537267No Hit
CTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGC7750.1159587695502745No Hit
GACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGG7660.11461215158130354No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7630.1141632789249799No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7610.11386403048743081No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7600.11371440626865627No Hit
CCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTC7560.11311590939355808No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7550.11296628517478352No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7540.11281666095600898No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG7520.11251741251845988No Hit
GATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACA7490.11206853986213625No Hit
GATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAG7490.11206853986213625No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAA7380.11042267345561622No Hit
CCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGA7380.11042267345561622No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7350.10997380079929259No Hit
AGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTC7330.10967455236174349No Hit
GTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGC7310.1093753039241944No Hit
GTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG7310.1093753039241944No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAAT7290.1090760554866453No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7250.10847755861154709No Hit
CACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTG7080.10593394689237978No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7070.10578432267360524No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGCGTGGAGT6970.10428808048585977No Hit
GTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATA6920.10353995939198703No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG6910.10339033517321249No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG6900.10324071095443793No Hit
GCCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT6900.10324071095443793No Hit
ATACAAATACGTCTCTCCATCACTATGCAGGAGGCTCTGACTAGACCTGC6820.10204371720424153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)