FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668341
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46220.6915631391759596No Hit
GTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAA31520.4716155375773744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27410.4101199836610353No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26820.401292154753337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25870.38707785396975497No Hit
GTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCT25140.37615528599921294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25010.3742101711551439No Hit
GTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTT24320.36388610005970007No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA22160.33156726880439774No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21710.32483417895954314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21440.32079432505263034No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGACCAAATGCG20430.305682278956401No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19690.29461008676708444No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC18670.2793484164520806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18500.2768048047329133No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG16830.25181756019756385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16530.24732883363432737No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16380.2450844703527092No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTC15890.23775288363275635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15730.23535889613236355No Hit
GACCAAATGCGTACGGGGCCCGTATACTTTCCATGCAGTAATAAACCCCA15350.22967317581893076No Hit
CATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15270.22847618206873438No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15220.22772806097486162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15110.2260821945683416No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT14910.22308971019285062No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14870.22249121331775246No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14730.2203964742549088No Hit
GTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT14270.2135137601912796No Hit
GAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14220.21276563909740687No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGA14160.21186789378475956No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA14020.2097731547219159No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG13930.20842653675294498No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13680.20468593128358126No Hit
GGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGG13670.20453630706480674No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13170.19705509612607933No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12900.19301524221916655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12510.1871798976869592No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12390.18538440706166462No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12090.1808956804984282No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11980.17924981409190818No Hit
GACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCT11920.17835206877926088No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11900.1780528203417118No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGT11770.17610770549764265No Hit
CTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT11610.1737137179972499No Hit
AGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT11450.17131973049685714No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11290.16892574299646437No Hit
GCCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT11290.16892574299646437No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCT11280.16877611877768983No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACG11110.16623250705852252No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11000.1645866406520025No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10970.16413776799567883No Hit
CCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTC10810.1617437804952861No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10520.1574046781508242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10490.15695580549450056No Hit
CCCGTATACTTTCCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAAT10370.15516031486920598No Hit
CTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAG10230.15306557580636232No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10120.15141970939984228No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGAC10080.1508212125247441No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10070.15067158830596955No Hit
GTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATG10030.15007309143087136No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9930.1485768492431259No Hit
GTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA9810.14678135861783131No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9730.14558436486763493No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9730.14558436486763493No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCC9720.1454347406488604No Hit
GTGTGAAATCTGTCTCTGACCCGCTGCCACTGAACCTATCTGGCACTCCA9560.14304075314846762No Hit
GTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGG9420.14094601408562396No Hit
GATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGGAAAGTATACGGGCC9340.13974902033542758No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9180.1373550328350348No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9170.13720540861626027No Hit
GCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCC9080.13585879064728934No Hit
GCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCC8950.13391367580322022No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8820.13196856095915108No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA8740.13077156720895472No Hit
CCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCC8740.13077156720895472No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8710.13032269455263107No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8710.13032269455263107No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAATACGTCTCTCCA8560.12807833127101287No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8390.12553471955184553No Hit
CTCCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC8350.12493622267674735No Hit
GTCTCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGGCT8290.12403847736410006No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8250.12343998048900187No Hit
TCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACA8250.12343998048900187No Hit
CCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAG8220.12299110783267823No Hit
GCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGT8190.1225422351763546No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8140.12179411408248185No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7970.11925050236331453No Hit
TCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGG7970.11925050236331453No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7940.11880162970699089No Hit
ACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTT7900.1182031328318927No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7890.11805350861311815No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7810.11685651486292177No Hit
ATCTGGCACTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA7720.11550989689395084No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7670.1147617758000781No Hit
ATATTGTGCTGACCCAGACTCCACGCTCTCTGTCCGTCACCCCTGGACAG7650.114462527362529No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7520.11251741251845988No Hit
CTCCAGAGAACCGGCTGGAAAGTTCATAGATCAGGAGCTGTGGAGGCTGG7520.11251741251845988No Hit
TCCTCAGCCTCCACCCGACTGATTGTCAGTGTGAAATCTGTCTCTGACCCGCTGCCACTGAACCTATCTGGCACT7490.11206853986213625No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAC7470.11176929142458715No Hit
GGTCAGAGACAGATTTCACACTGACAATCAGTCGGGTGGAGGCTGAGGAT7440.11132041876826351No Hit
TCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG7410.11087154611193986No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7390.11057229767439077No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7380.11042267345561622No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGCTGACCCAGACTC7360.11012342501806713No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7360.11012342501806713No Hit
CTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGT7350.10997380079929259No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7250.10847755861154709No Hit
GTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAACTTTCCAGCCGGTTCT7180.10743018908012526No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7140.10683169220502707No Hit
TCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT7130.10668206798625252No Hit
AGTCAGAGCCTCCTGCATAGTGATGGAGAGACGTATTTGTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAG7040.1053354500172816No Hit
GACAATCAGTCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGG7000.10473695314218341No Hit
GTTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAA6960.10413845626708522No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6960.10413845626708522No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCAC6960.10413845626708522No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6940.10383920782953612No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6920.10353995939198703No Hit
GTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG6850.1024925898605652No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATACAAAT6800.10174446876669244No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6770.1012955961103688No Hit
GGTTTATTACTGCATGGAAAGTATACGGGCCCCGTACGCATTTGGTCAGGGGACCAAGCTGGAGATCAAACGAA6770.1012955961103688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)