FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985959
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39050.3960610938183028No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27520.27911911144378215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24110.24453349480049374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23430.23763665629098168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23160.23489820570632247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22510.22830563948399474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21360.21664186847526115No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA20240.2052823697537119No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20220.20507952156225565No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18660.18925736262866913No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18590.1885473939585723No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17250.17495656513100444No Hit
GCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT17180.17424659646090762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17170.17414517236517948No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCAC17170.17414517236517948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16760.16998678444032664No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16380.16613266880265815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16190.16420561098382389No Hit
GACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16070.16298852183508644No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15990.16217712906926152No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15900.16126431220770843No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15750.15974295077178666No Hit
ACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15720.1594386784846023No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15530.15751162066576804No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC15370.15588883513411814No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA15130.1534546568366433No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15110.15325180864518706No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14700.1490934207203342No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13890.14087806896635663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13610.13803819428596928No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13410.1360097123714069No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12510.12688154375587626No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12480.1265772714686919No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12330.12505591003277014No Hit
GTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGA12260.12434594136267331No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT12000.1217089148737422No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA11870.12039040162927668No Hit
GATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11690.11856476790617054No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA11640.11805764742752994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11420.11582631732151133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11350.11511634865141451No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11170.11329071492830838No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11160.11318929083258027No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11060.11217504987529907No Hit
TTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTC10860.1101465679607367No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10510.10659672461025256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10250.10395969812132147No Hit
CTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTT10230.10375684992986524No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10210.103554001738409No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9930.10071412705802169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA250.00204631871.4877570
GTATCAA25000.037.774061
CGTATGC1250.036.01874541
TCGTATG1400.034.60685340
CTCGTAT1300.034.58917239
TTAACGG709.802043E-734.46942535
TATCAAC27700.033.7184872
ATCAACG28400.032.7664873
CGCGGTA751.5974911E-632.38083343
GTGGTAT3500.032.3777661
TCAACGC28800.032.3113984
CAACGCA28800.032.1921655
AACGCAG29200.031.8687766
GCGTGTA652.359783E-531.7694132
CTTAACG450.00419996731.2731758
ACGAACC853.9833158E-628.8000752
ACGCAGA32400.028.7183287
TAACGGC854.4385397E-628.40249436
CGCAGAG33000.028.1961788
AGAGTAC33200.027.92290511
Produced by FastQC (version 0.11.5)