FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985959
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38310.388555710734422No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24380.24727194538515293No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23810.24149077192865018No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23430.23763665629098168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22460.22779851900535417No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22270.22587146118651993No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCACCTATTTAAATTGGTATCAGCAGAAA19000.1927057818834252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18810.19077872406459093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17700.17952064943876975No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17570.17820213619430422No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAGCATTAGCAC16810.1704939049189672No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16240.16471273146246446No Hit
GTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA16160.1639013386966395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16070.16298852183508644No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15990.16217712906926152No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15910.16136573630343654No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15740.15964152667605852No Hit
GACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15670.1589315580059617No Hit
ACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15420.15639595561275876No Hit
GCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT15350.15568598694266192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15300.15517886646402132No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14970.1518318713049934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14910.1512233267306247No Hit
GATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA14720.14929626891179046No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14630.1483834520502374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14530.1473692110929562No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14480.1468620906143156No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14340.14544215327412194No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC14050.1425008544980065No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13690.13884958705179423No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13390.1358068641799507No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13000.13185132444655406No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12520.12698296785160437No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12400.12576587870286696No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12200.12373739678830457No Hit
GTCTGCAACCTGAAGATCTTGCAACTTACTACTGTCAACAGACTTACAATATGGCGCTCACTTTCGGCGGAGGGA11920.12089752210791727No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11700.11866619200189867No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA11690.11856476790617054No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11640.11805764742752994No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11480.11643486189588007No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCGTCAGCAGTCTGCAACCTGAAGATCTTGCAACTTA11380.11542062093859887No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11330.11491350045995828No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11020.11176935349238659No Hit
CTCCTGATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTT10910.1106536884393773No Hit
GATCTATGACGCATCCAGATTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA10830.10984229567355235No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10630.10781381375898998No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10620.10771238966326185No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC10420.10568390774869948No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10290.10436539450423396No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10230.10375684992986524No Hit
TTGTAAGTCTGTTGACAGTAGTAAGTTGCAAGATCTTCAGGTTGCAGACTGCTGACGGTGAGAGTGAAATCTGTC9910.10051127886656545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.040.917281
TATCAAC27500.035.8118972
ATCAACG27750.035.5876273
CGTTTAG400.00226922535.45967563
TCAACGC28400.035.1193054
CAACGCA29050.034.330025
GTGGTAT3700.033.4278531
AACGCAG30000.033.24296
AGCGATA450.004282741331.148753
ACGCAGA32450.030.838657
AGAGTAC32900.030.31267711
CGCAGAG33250.030.1997388
GCAGAGT34650.028.9795469
CGGTGTA500.00780567227.54814734
GAGTACG24800.027.3615512
AGTACGG24950.026.92233513
CAGAGTA38150.026.32087110
TACGGGG13000.024.78310615
TGTTAGA8450.024.50794870
GTACGGG27600.024.0890614
Produced by FastQC (version 0.11.5)