FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577406
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6330.1096282338597105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT704.774069E-543.45555570
GTGGTAT2550.040.5610051
GTATCAA18450.040.5500141
GGTATCA7600.037.198711
TATCAAC20950.035.4879842
ATCAACG21300.035.2036173
TCAACGC21450.035.0995454
TATACGA400.002572909734.56089836
CAACGCA21700.034.533765
TGGTATC3000.034.4200552
AACGCAG22600.033.614836
CGCAGAG24000.031.7971948
ACGCAGA23900.031.7864077
AGAGTAC24250.031.32763511
CAGAGTA24850.030.98622710
TTAACGG901.9721847E-730.7020935
GCAGAGT25300.030.1633479
TAACGGC953.1500713E-729.10391636
ACGGGTA1057.906692E-726.19527816
TCGATTG800.001067519925.5988569
Produced by FastQC (version 0.11.5)