FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694841
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23900.042.6602861
GGTATCA9150.042.5390051
ATCAACG29250.035.328513
TCAACGC29250.035.210754
CGTTAAG400.00241025835.025156
TATCAAC29900.034.44532
CAACGCA30150.034.0454375
AACGCAG30350.033.9345786
GTGGTAT3050.033.8806231
ACGCAGA32350.031.725577
CGCAGAG33250.030.8668358
AGAGTAC34000.030.28724511
CAGAGTA34750.029.6335610
GCAGAGT35600.028.9260189
AGTACGG23750.028.85738413
GAGTACG24100.028.72410412
GTTAACG500.006747427428.381566
TACGGGG10350.027.62081315
CGTGCGT500.00779842827.55227510
CGGTACC2150.027.5270645
Produced by FastQC (version 0.11.5)