FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694841
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24400.041.696981
GGTATCA9000.041.3860171
TATCAAC27900.036.2980352
TCAACGC29150.034.4579934
ATCAACG29650.034.0100673
CAACGCA29800.033.703975
AACGCAG30300.033.1477976
AGAGTAC31700.031.57535211
ACGCAGA32150.031.2403817
CGCAGAG32600.030.8091498
CAGAGTA32850.030.6793910
GCAGAGT33500.030.3921479
GTGGTAT3900.028.2981321
TGGTATC3550.028.1390612
GAGTACG24350.027.40420512
AGTACGG24450.027.29212413
TACGGGG11700.025.87274715
GTACGGG27800.023.75586914
GAGTACT14050.023.01261712
AGTACTT14500.022.53564813
Produced by FastQC (version 0.11.5)