FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG305.491111E-447.31397666
GGTATCA7950.043.7175561
GTATCAA17150.038.7253071
GTGGTAT3100.033.3013041
TATCAAC20400.032.387152
TCAACGC20600.032.0727124
ATCAACG20800.031.7643183
CAACGCA21500.030.7301335
AACGCAG22250.029.6942846
ACGCAGA23050.028.8129737
AGAGTAC23400.028.52906411
TGGTATC3500.028.512262
CGCAGAG23900.027.9322228
CAGAGTA24250.027.52907810
GCAGAGT25250.026.7113829
AGTACGG20450.024.40130813
GAGTACG21250.023.4826712
TTTCGAT902.1582926E-422.94671824
GTAGTTT2754.9665687E-821.84088170
GTACTTT7800.021.61921114
Produced by FastQC (version 0.11.5)