FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591386
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA200.00743791851.6075115
GTATCAA16700.042.5368161
GGTATCA6400.038.2553861
ATCAACG18500.037.752523
TCAACGC18950.036.674464
CAACGCA19200.036.0177425
TATCAAC19650.035.9236372
AACGCAG20000.034.577036
ACGCAGA20950.033.0090987
AGAGTAC21300.032.9512711
GTGGTAT2900.032.105541
CGCAGAG21700.031.868238
CAGAGTA22350.031.2492910
TGGTATC2900.030.871972
GCAGAGT23750.029.2623629
ACGCTAC500.00728669427.93738452
AGTACGG21150.027.65414213
GAGTACG21150.027.4914712
GTAGTTT2357.91988E-925.96376470
GTACGGG23950.024.13378314
Produced by FastQC (version 0.11.5)