FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39015
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT6411.6429578367294628No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT1080.27681660899653976Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT890.2281173907471485Illumina PCR Primer Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT770.19735998974753302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG900.069.18986570
ATCTGGT150.002322301369.11655437
CATGGAC150.00264314866.9012811
GTACAAC150.00264314866.901281
GCGTGAA200.00624658153.83390456
GGATGAT200.0064534353.3932451
ACGGCAA350.006423619653.3750470
TACACCT200.00756511651.29390332
CAGAGAA409.039577E-750.89369229
GCAGAGA409.3702147E-750.63033728
CCATGGA200.0082550350.17595710
AGTACCG200.0082550350.1759575
TTATACA1100.048.6554762
CTTATAC1100.048.6554761
GACAAAA305.6359934E-446.9482743
GAGAAAA305.673687E-446.88542
GGACAAA305.673687E-446.88542
TATACAC1150.046.5400243
TACACAT1150.046.5400245
ATACACA1200.044.6008574
Produced by FastQC (version 0.11.5)