FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628449
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18150.28880625158127393No Hit
GAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT17430.27734947465904153No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15530.24711631333648393No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15160.24122880297367008No Hit
GTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCA13570.21592842060374035No Hit
CTCCTGATCTGTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT13510.2149736891935543No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13500.2148145672918566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13340.2122686168646939No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12750.20288042466453127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12450.19810676761360108No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12100.1925375010541826No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGAT11060.17598882327762475No Hit
ACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGA10980.1747158480640434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10770.17137428812839228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10090.16055399881295063No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10000.15912190169767157No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC9940.15816717028748553No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9900.15753068268069484No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9380.14925634379241592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9260.14734688097204388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9100.14480093054488113No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9090.14464180864318346No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8940.14225498011771837No Hit
ATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC8650.1376404449684859No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8540.1358901040498115No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8530.13573098214811385No Hit
ATGCAGCACAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGATAGATATTAATGCT8230.1309573250971837No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8060.12825225276832328No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8030.12777488706323029No Hit
GGGTGATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT7540.11997791388004436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7500.11934142627325367No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7420.11806845105967231No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7400.11775020725627695No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC7240.11520425682911423No Hit
CCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7020.11170357499176543No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC6850.10899850266290502No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6780.10788464935102132No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAT6750.1074072836459283No Hit
GTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA6730.10708903984253297No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACC6610.10517957702216091No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6550.10422484561197487No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATATCTATCTAAACTGGTATCAGCAGAAA6550.10422484561197487No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6480.10311099230009117No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6370.10136065138141678No Hit
TCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA6310.10040591997123076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)