FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354724
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200.007270716651.90078739
TATCGGC200.0073729551.71785723
GGTATCA3400.042.597181
GTATCAA8150.041.8878481
ATCAACG9600.034.8377233
TCAACGC9600.034.8377234
AACGCAG9600.034.8328136
TCGATTA400.002516604534.71348644
ATCGATT400.00252186634.6987143
AGTCTAA400.002603639834.4737110
CAACGCA9800.034.1267475
TATCAAC10000.033.7889982
CGTGTGC450.004632486530.6476173
AGAGTAC11050.030.57396911
ACGCAGA11000.030.3995447
TGGTATC1301.9826984E-1029.1741732
CAGAGTA11700.028.87541610
CGCAGAG11600.028.8271548
GTGGTAT1202.6848284E-928.7361951
GCAGAGT11850.028.5099039
Produced by FastQC (version 0.11.5)