FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354724
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.042.336321
GGTATCA4150.039.8846631
TATCAAC11400.037.487312
AACGCAG11950.035.130596
ATCAACG12150.034.865013
TCAACGC12100.034.6999744
GTGGTAT1400.034.4836161
GGTTATG501.887358E-434.4106987
CAACGCA12250.033.9893465
GTATTGG851.209446E-732.4551661
ACGCAGA13050.032.1693887
AGAGTAC13500.030.33239411
CGCAGAG13750.030.2814148
TGGTATC1501.8189894E-1229.8689212
CAGAGTA13850.029.81432510
GCAGAGT14600.028.7541459
GTATACC605.429792E-428.7363431
ATGTACG500.00782618327.52855911
GAGTACG12850.026.77875112
AGTACGG12850.026.51096513
Produced by FastQC (version 0.11.5)