FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255983
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39220.3122653730185838No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29240.2328056988032481No Hit
ACTTATTACTGTGCGAGACATGTCCGATTCTCCTTCGATTTTACTTACAT28950.2304967503541051No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27770.2211017187334542No Hit
GTAATAAGTAGCCGTGTCTGCGGCGGTCACAGCGCTCAGATTCAGGGAGA27050.21536915706661633No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26490.21091049799240913No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25820.20557603088576837No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24440.19458862102432914No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24030.19132424563071315No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23630.18813948914913659No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATT23610.18798025132505775No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23170.1844770191953235No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22490.17906293317664332No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22240.17707246037565794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22170.17651512799138205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21410.17046409067638654No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20680.1646519100975093No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20210.16090982123165679No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20040.15955629972698673No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19020.15143517069896648No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18710.14896698442574463No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG18610.14817079530535046No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18610.14817079530535046No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18210.1449860388237739No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17970.14307518493482796No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17760.14140318778200023No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17750.14132356886996084No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17660.1406069986616061No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTCTC17460.1390146204208178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17010.13543176937904414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16930.13479481808272883No Hit
CTATAGTCCCTTTGCCCCAGACGTCCATGTAAGTAAAATCGAAGGAGAAT16810.13383939113825585No Hit
GTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCCCTCCGCCGAAAGAG16410.13065463465667926No Hit
ACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCC16270.12953996988812747No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16040.12770873491122092No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15970.127151402526945No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG15790.12571826211023557No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15680.12484245407780202No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15570.12396664604536844No Hit
GACTTCACTCTCACCATCAGTAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTAGCAACTGG15520.12356855148517139No Hit
GTTTATTACTGTCAACAGCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGG15480.12325007583701372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15410.12269274345273781No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA15230.12125960303602835No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15100.12022455717951597No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15010.11950798697116123No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGGATCGGAGAGGGCCACTGGCGTCCCAGCCAGGTT14850.1182340843785306No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14590.11616399266550581No Hit
GGACTATAGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCA14580.11608437375346642No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA14300.1138550442163628No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14280.11369580639228397No Hit
ACCTACTACATCCCGTCCCTCAAGAGTCGAGTCACCATATCCCTTGACACGTCCACGAATCACTTCTCCCTGAAT14230.1132977118320869No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14200.11305885509596866No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATGGAGCTCCACCTTGGTCC13920.11082952555886505No Hit
CTCTTATAGTGGGACCACCTACTACATCCCGTCCCTCAAGAGTCGAGTCA13790.10979447970235266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13790.10979447970235266No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA13780.10971486079031324No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13670.1088390527578797No Hit
GACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCATCTGTCTTCATCT13440.10700781778097315No Hit
GCGTAGCAACTGGCCGGAGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGCTCCATCGAACTGTGGCTGCACCAT13420.10684857995689433No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13310.10597277192446075No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13210.10517658280406661No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13110.10438039368367248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13070.10406191803551482No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12980.10334534782716008No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12690.10103639937801706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA150.002398556368.68622615
CGTACTA200.007495363751.51056714
CGTATGC1800.044.3293941
GTATCAA21250.043.3196261
TCGTATG1900.041.9507140
CTCGTAT1950.040.84551239
GGTATCA7900.040.4356771
ATGCCGT1900.040.2318344
AAGGGAT11850.040.16232770
ACGAGAC2000.039.53708322
TATCAAC24050.038.1303182
TATACAC2350.037.999663
CTTATAC2250.036.638491
ATCAACG25450.036.1677323
TCAACGC25700.035.8159034
AATCTCG2050.035.38219536
TACACAT2650.034.9938855
CAACGCA26400.034.866245
CGTCGAG400.002641140534.3800721
AACGCAG27350.033.6551676
Produced by FastQC (version 0.11.5)