FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411393
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45250.035.8604971
GGTATCA19800.034.830411
TCAACGC52150.031.5684244
ATCAACG52350.031.4478173
CAACGCA52900.031.1208575
TATCAAC54050.030.6463432
AACGCAG54150.030.2776056
ACGCAGA57850.028.4569577
AGAGTAC58900.027.77748711
CAGAGTA59750.027.438910
CGCAGAG60200.027.3460968
GCAGAGT62200.026.3037599
AGTACGG45650.026.06634313
GAGTACG46950.025.34459312
GTGGTAT8650.024.230881
TACGGGG21000.023.34305815
GTACGGG50400.023.2743314
TAGTTAG1400.002136137822.72600470
TACGGGC9400.022.65800915
TTAACGG2950.022.0127835
Produced by FastQC (version 0.11.5)