FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411393
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46700.035.839061
GGTATCA21600.033.2528341
GTGGTAT10100.031.8675611
TATCAAC53600.031.0648292
ATCAACG54350.030.2947123
TCAACGC54600.030.2104284
AACGCAG55250.029.9709916
CAACGCA55300.029.881735
ACGCAGA58950.028.1471887
AGAGTAC61150.027.30032511
CGCAGAG61950.026.8932298
CAGAGTA62900.026.70195810
AGTACGG50200.026.38786913
TGGTATC11700.026.3223192
GAGTACG51050.025.94941912
GCAGAGT64450.025.7976139
AATCGTA1300.001347813924.5974570
TACGGGG23900.024.179715
GTACGGG54950.023.92235214
TACGGGT10250.022.09040515
Produced by FastQC (version 0.11.5)