FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367232
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40950.039.187421
GGTATCA17450.036.352231
TATCAAC46650.034.290542
ATCAACG46950.034.037773
TCAACGC47350.033.517474
CAACGCA48350.032.751115
AACGCAG49000.032.246866
GTGGTAT7450.032.2178461
AGAGTAC53300.029.83783111
ACGCAGA53350.029.6816527
CAGAGTA54500.029.118110
CGCAGAG54550.029.091418
GCAGAGT56850.027.9144489
TGGTATC8100.027.487642
AGTACGG44050.025.77707913
GAGTACG44550.025.56454312
TACGGGA13000.023.4163915
GTACGGG48600.023.22304514
GAGTACT20650.022.5247712
TACGGGT12800.021.64453915
Produced by FastQC (version 0.11.5)