FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537528
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC25030.46565016147996013No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCA24750.46044113050855023No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21800.40556026848833926No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16410.3052864222886994No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAG15840.29468232352547213No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15600.2902174398356923No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC14930.27775297286839007No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGG14300.2660326531827179No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14190.2639862481582355No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13900.25859118036641815No Hit
GTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCG13240.24631275021952345No Hit
GTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA12540.2332901727909988No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC12490.23235998868896135No Hit
GGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT12300.2288252891012189No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12220.22733699453795897No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12110.22529058951347652No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11700.2176630798767692No Hit
CCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG11530.2145004539298418No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11180.20798916521557945No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10830.20147787650131713No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAG10200.18975755681564496No Hit
CATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCT10180.18938548317482998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10090.18771115179116252No Hit
GACTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGG9940.18492059948505007No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9780.18194401035853017No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9450.1758047952850828No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9330.17357235344019287No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9260.17227009569734042No Hit
GGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGG9230.17171198523611791No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9230.17171198523611791No Hit
TCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAGCA9020.16780521200756054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8730.16241014421574318No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT8630.16054977601166823No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8560.15924751826881578No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8510.1583173341667783No Hit
ATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTT8430.15682903960351832No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA8400.15627092914229584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8390.15608489232188832No Hit
CTCCAGCGATGGTCAAAGTGACTGTTGTTCCTGAACTGGAGCCAGAGAATCGCCCAGGGATCCCTTGAGGCCTCT8370.15571281868107337No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8350.15534074504025835No Hit
CCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCT8310.15459659775862838No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8300.1544105609382209No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8000.14882945632599603No Hit
CTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATA7980.14845738268518105No Hit
GCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGG7960.14808530904436606No Hit
CACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGACCATCG7650.14231816761173371No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7510.13971365212602876No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7430.13822535756276882No Hit
CACCAATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGA7400.13766724710154635No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7360.13692309981991638No Hit
GCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTT7360.13692309981991638No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7280.1354348052566564No Hit
GCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA7200.13394651069339644No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7180.13357443705258146No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC7060.1313419952076915No Hit
GTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACA7020.13059784792606152No Hit
GGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCT6950.12929559018320907No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6940.12910955336280155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6860.12762125879954161No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6780.12613296423628165No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT6750.12557485377505917No Hit
CAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGT6670.1240865592117992No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6650.1237144855709842No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6640.12352844875057672No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAG6590.12259826464853923No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6550.12185411736690926No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6400.11906356506079684No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6390.11887752824038934No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6340.11794734413835187No Hit
GGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTTGGCAAAGCATCTCCAGAGCAGGTGATCCTGGC6320.11757527049753687No Hit
GTTCAGGAACAACAGTCACTTTGACCATCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAG6270.1166450863954994No Hit
AGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTT6260.11645904957509191No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6230.11590093911386942No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6210.11552886547305442No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC6200.11534282865264694No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT6160.11459868137101695No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5960.11087794496286706No Hit
GGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCC5910.10994776086082957No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5780.10752928219553214No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5720.10641306127308717No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5700.10604098763227218No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5680.10566891399145718No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC5680.10566891399145718No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5670.1054828771710497No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTAT5650.1051108035302347No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5610.10436665624860472No Hit
CTCCACTGCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC5480.10194817758330729No Hit
GCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT5420.10083195666086232No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5400.10045988302004732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)