FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536235
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65440.4259764944816386No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCT54030.3517040036192379No Hit
GTCTATTACTGTGCGAGACAACGGGTCTCGGGAACTCGGGGAGTCCCTGT52960.34473892340690065No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTCGCAGACCTCAGCTTCAGGGAGA48720.3171389793879192No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47250.3075701308719043No Hit
GCTTAGGAGGCTGACCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACACT46360.30177674639622193No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41690.27137775145078713No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41410.26955511363821294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40830.26577964959788053No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40490.2635664465397547No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40170.26148343189681267No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38580.25113345288969463No Hit
GTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGA36280.23616178514354902No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36220.23577121989799738No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT35470.23088915432860208No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATA34340.22353350887071313No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC34310.2233382262479373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33800.2200184216607485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33680.2192372911696453No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33260.2165033344507839No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30020.19541281119099616No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29740.1935901733784219No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29240.19033546299882506No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29030.18896848463939436No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28970.18857791939384275No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC26610.1732156864021455No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26540.17276002694900194No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26020.1693751281542212No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24710.1608477869596774No Hit
GTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA24670.16058741012930966No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24540.15974118543061444No Hit
GTATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGG24500.15948080860024672No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24490.15941571439265476No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24360.1585694896939596No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24340.1584393012787757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24060.15661666346620146No Hit
CTCCAGAGCCGAGTCACCATATCCGTAGACACGTTCAAGAATCAGTTCTC23790.15485911986121917No Hit
CTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAAAACAC23680.15414308357770784No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA23620.15375251833215622No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23590.1535572357093804No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA23570.1534270472941965No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCACCAATAGGAACTA23400.1523204457651336No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23010.14978177166904802No Hit
GCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCGGGGGTCCCTGACCGATT22630.1473081917805544No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22520.1465921554970431No Hit
GTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22480.14633177866667535No Hit
GTTTTATTACGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAG22440.1460714018363076No Hit
ATTTTAGTACTCCCCCCTCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22260.14489970609965272No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22180.14437895243891724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22170.14431385823132528No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22070.1436629161554059No Hit
GACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA21950.14288178566430268No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21940.14281669145671072No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTACGCTCCAC21920.14268650304152683No Hit
CACCTGCACTGTCTCCGGTGACTCCATCAGGAGTAATAATTACTACTGGG21910.14262140883393493No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21750.1415799015124639No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21680.14112424205932037No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT21500.13995254632266546No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21340.13891103900119448No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21040.13695821277343634No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20600.1340940676393911No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG20550.13376859660143142No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20460.13318274873310398No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT20250.1318157703736733No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20230.13168558195848942No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20200.1314902993357136No Hit
GTCTCCGGTGACTCCATCAGGAGTAATAATTACTACTGGGGCTGGATCCG20050.13051388622183455No Hit
CCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATTTCCTGGGCAT20010.1302535093914668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19960.1299280383535071No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC19820.12901671944721999No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA19490.12686861059668605No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTTTAGTACTCCCCCCTCGAT19410.12634785693595055No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19400.12628276272835862No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19230.1251761611992957No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTT18730.12192145081969881No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18680.12159597978173914No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17940.11677900841993574No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17870.1163233489667922No Hit
GGCCGAAGGTGATCGAGGGGGGAGTACTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG17740.11547712426809702No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA17700.11521674743772926No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17500.1139148632858905No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17450.11358939224793083No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17120.11144128339739688No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17010.11072524711388558No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17010.11072524711388558No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16990.1105950586987017No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16650.10838185564057583No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16610.10812147881020809No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16610.10812147881020809No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16480.10727525411151288No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGTCAGCCTCCTAAGCTGCTCATT16420.10688468886596127No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC16380.10662431203559353No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16320.1062337467900419No Hit
GTCAGGGACCCCCGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGACCTGGTCTCTGCTGGTA16300.10610355837485802No Hit
CTAAAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC16280.10597336995967414No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16270.1059082757520822No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16230.10564789892171444No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16210.10551771050653057No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16060.10454129739265151No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15870.10330450744840469No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15740.10245828274970951No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCGTAATAA15510.10096111597509495No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC15390.10017998548399171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT12250.054.6401770
GTGGTAT2950.049.130111
TGGTATC3050.045.2564662
GGTATCA9300.042.671251
GTATCAA22350.040.7611921
TATCAAC24900.036.4482652
CTACGGA12800.035.2768869
TAAGCGC400.002519152134.71368847
GTCTATC400.002593634934.5080571
ATCAACG26400.034.246633
TCAACGC26450.034.181894
CAACGCA26650.034.054855
AACGCAG27100.033.489376
CGCAGAG28700.031.8628378
AACGTGT12600.031.11949262
GTGTCTA12600.030.96638765
GCATTAG450.00461789530.67382613
CAGAGTA29650.030.49278510
ACGTGTC12800.030.39084663
AGAGTAC29700.030.32526211
Produced by FastQC (version 0.11.5)