FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930812
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.040.320191
GTATCAA26950.039.7611851
TATCAAC30750.034.8457372
GGTATCA12200.034.1730081
ATCAACG31100.034.121233
TCAACGC31400.033.7952354
CAACGCA31550.033.6345565
TGGTATC5750.033.554792
AACGCAG32350.032.802796
AACGTTA750.00397319331.62737370
AGAGTAC35450.029.73511311
ACGCAGA35850.029.5971077
CGCAGAG36100.029.4875688
CAGAGTA36900.028.66001310
AGTACGG28950.028.20097713
GAGTACG29150.028.00748812
GCAGAGT39250.027.0318289
TACGGGT5200.025.8360215
GTACGGG32500.025.01456614
TACGGGA10150.024.77542915
Produced by FastQC (version 0.11.5)