FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930812
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26350.042.2408871
GGTATCA10800.039.883881
TCAACGC30300.036.3109364
TATCAAC30550.036.0622252
ATCAACG30550.036.0292743
CAACGCA30600.035.951085
AACGCAG31050.035.3193326
TATACGA400.002479943434.8234346
AGAGTAC34200.032.1667611
ACGCAGA34550.031.9404057
CGACCCG952.9546424E-431.885170
CGCAGAG35000.031.6279668
CAGAGTA35250.031.20859710
GCAGAGT36450.030.3697859
TAACGAG800.00490768630.29084870
GTGGTAT4000.030.1522121
CGATATC605.1332574E-429.01952646
AGTACGG28000.028.36203613
GAGTACG28800.027.4548312
TACGGGG15350.024.63718415
Produced by FastQC (version 0.11.5)