FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826232
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC42690.5166829655593101No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39450.47746879810997395No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39050.47262754286931513No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT35190.4259094297969578No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33350.40363965568992727No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30540.36962983762429924No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28600.34614974970710405No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28410.34385015346779113No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26620.322185536265843No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG26320.3185545948353489No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25150.30439392325642195No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24310.2942272872510384No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA24060.2912015027256267No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23530.28478683953175377No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23390.28309240019752324No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTA23210.28091383533922676No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT23010.27849320771889735No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCA22870.27679876838466677No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGAC22230.2690527599996127No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG21250.25719168465999864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21090.25525518256373514No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT20980.253923837372554No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20250.24508854655835166No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20070.2429099817000552No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT20020.24230482479497287No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTC19360.2343167536478858No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC18750.22693383940588116No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18240.2207612389740412No Hit
GATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTA18210.22039814483099177No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18020.21809854859167885No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA17970.2174933916865965No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17920.21688823478151417No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17620.21325729335102006No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17570.2126521364459377No Hit
GTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTT17170.2078108812052789No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17130.20732675568121303No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC16900.2045430339178342No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG16750.20272756320258717No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTTCTGTGCAG16290.19716011967582953No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTC16240.1965549627707472No Hit
CCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA15970.19328711548330252No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGT15560.18832482886162724No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15290.18505698157418254No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG14690.17779509871319435No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14640.177189941808112No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14140.17113837275728852No Hit
GTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAG14120.17089630999525557No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14100.17065424723322262No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14040.16992805894712382No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC14020.16968599618509086No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCT13950.16883877651797558No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAGCTCCCAGGGGCGGCCCC13700.16581299199256383No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13670.16544989784951442No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13270.16060864260885563No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13140.1590352346556415No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13050.15794595222649327No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12990.15721976394039447No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12900.15613048151124626No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12770.15455707355803214No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12750.15431501079599919No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12710.1538308852719333No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12650.15310469698583448No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGG12450.1506840693655051No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG12380.14983684969838978No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAAC12270.14850550450720865No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12210.14777931622110982No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC12150.147053127935011No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG12060.14596384550586275No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11800.14281702959943454No Hit
GGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG11780.14257496683740162No Hit
GATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAG11680.1413646530272369No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGG11650.1410015588841875No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC11490.13906505678792397No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTC11380.1377337115967428No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11180.1353130839764134No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11130.13470792707133106No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11060.13386070740421577No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11010.1332555504991334No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC10970.13277142497506755No Hit
CTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10830.13107698564083697No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTA10790.13059286011677107No Hit
GGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC10660.12901945216355698No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10630.12865635802050754No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10460.12659882454322757No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10380.1256305734950958No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10290.12454129106594758No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG10290.12454129106594758No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG10240.12393613416086521No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10170.12308891449374995No Hit
GTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA10080.1219996320646017No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10020.1212734437785029No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9990.12091034963545347No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9740.11788456511004172No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9690.11727940820495938No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9680.1171583768239429No Hit
GACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT9640.11667425129987703No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9590.11606909439479468No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC9250.11195402744023471No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA8950.1083230860097406No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT8790.10638658391347708No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA8750.1059024583894112No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8720.1055393642463618No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA8680.10505523872229591No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAG8640.10457111319823005No Hit
CAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG8550.1034818307690818No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8550.1034818307690818No Hit
CTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGC8490.102755642482983No Hit
GTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCT8460.10239254833993357No Hit
GGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA8390.10154532867281828No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8360.10118223452976888No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT8340.10094017176773594No Hit
CGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT8340.10094017176773594No Hit
CTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT8310.10057707762468653No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC8300.10045604624367005No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG8270.10009295210062065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.043.5431821
GTATCAA16750.042.8967481
TGGTATC2100.042.7094462
GTGGTAT2200.042.3952261
TATCAAC19100.037.3858152
ATCAACG20500.034.969113
TCAACGC20750.034.354654
CAACGCA21100.033.9459425
TTCGGGG1756.7466317E-933.57448670
AACGCAG21500.033.4745566
CGCAGAG22850.031.4968478
ACGCAGA23050.031.2235577
GTATATA450.004593044530.7060051
GCTAGAC450.00464511630.6353323
TAAACCT450.004663036730.6112274
TATATGT450.004663036730.6112274
AGAGTAC23600.030.34997411
CAGAGTA23800.029.95024710
GCAGAGT24100.029.7203069
CGCGGTA605.27231E-428.8868443
Produced by FastQC (version 0.11.5)