FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685879
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT14780.2154899041959296No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG13330.19434914904815573No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGCTGTCCCATGTT12340.1799151162231239No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11950.17422898207992954No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG11220.16358570535036063No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10700.1560041931594348No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9950.145069319807138No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTACAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC9310.1357382278798447No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9290.13544663125711678No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9200.13413444645484116No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9160.13355125320938532No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACCGG8690.1266987325752793No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8560.12480335452754786No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8470.12349116972527223No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8410.1226163798570885No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8200.11955461531844538No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7920.11547226260025456No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7890.1150348676661627No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC7700.11226469975024748No Hit
GTGTATTACTGTGCGAGAGTTCGTATGGTTCGGGGAGTTACATTCCATTA7640.11138990988206375No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7340.10701596054114501No Hit
GTCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG7290.10628696898432523No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7170.10453738924795773No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7130.1039541960025019No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6980.10176722133204254No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6870.10016343990703899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1750.045.3293461
GGTATCA5350.044.4820671
TGGTATC1800.044.0669822
GTATCAA15100.043.8544731
TATCAAC18000.036.786352
ATCAACG18800.035.4044153
TCAACGC19000.035.0317344
GTATAGG807.105882E-834.489721
CAACGCA19450.034.2212375
AACGCAG19900.033.4473886
ACGGGAT1650.031.3497216
AGAGTAC21250.031.15565911
CGCAGAG21400.031.1006778
CAGAGTA21850.030.45794110
CTTATAC802.7659244E-630.1785031
GCAGAGT22350.029.7765549
TATAGGG703.8585276E-529.5604612
ACGCAGA22700.029.321727
CACGTCT500.007140696528.05409461
CTCGTAT756.0357965E-527.6826139
Produced by FastQC (version 0.11.5)