FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685879
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTCCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT15950.23254830662551265No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG14680.21403192108229002No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTACAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC12230.17831133479812036No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11390.16606427664354792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11240.16387730197308853No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCACCATCTCCTG9980.14550671474122986No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGCCAGAAGGTCAC9770.14244495020258674No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9430.13748780761621218No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGGCTTCTGGATACACCTTCACCGG9070.13223906840710972No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9020.13151007685028993No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGCTGTCCCATGTT8650.12611553932982347No Hit
GTGTATTACTGTGCGAGAGTTCGTATGGTTCGGGGAGTTACATTCCATTA8490.12378276634800016No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8360.12188738830026871No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8200.11955461531844538No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8070.11765923727071392No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG7790.11357688455252311No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7740.11284789299570333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7400.10789075040932876No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7370.10745335547523689No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7370.10745335547523689No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7310.10657856560705313No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7290.10628696898432523No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7190.10482898587068565No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7130.1039541960025019No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG7030.10249621288886232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.045.4296071
TGGTATC2300.044.989382
GTGGTAT2450.043.6970021
GGTATCA5650.040.9526861
TATCAAC19150.039.625112
ATCAACG20150.037.4600753
TCAACGC20150.037.4273034
AACGCAG20650.036.85466
CAACGCA20600.036.7768865
AGAGTAC21800.034.91043511
CAGAGTA21950.034.67186710
GTATCAG501.8496648E-434.534731
CGCAGAG22050.034.5146268
ATACTCC601.3587851E-534.436546
ACGCAGA22700.033.374627
GCAGAGT23400.032.8177269
TATAAGG450.00462673930.6594312
GCTAAAC450.004643290330.6370563
GAGTACG21900.029.5619612
AGTACGG21900.029.5619613
Produced by FastQC (version 0.11.5)