FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727045
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC37410.5145486180360226No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCA34410.473285697584056No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27800.3823697295215564No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22130.30438280986733973No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAG21550.2964053119132929No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21480.29544251043608033No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20930.2878776416865531No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC20610.28347626350501No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGG19700.27095984430124687No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19300.2654581215743179No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19250.2647704062334518No Hit
GTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCG18780.25830588202931043No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC18620.2561051929385389No Hit
GTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA17990.24743997964362588No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17440.23987511089409871No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17000.23382321589447697No Hit
CCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG16400.22557063180408365No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAG15430.21222895419128113No Hit
GGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT15430.21222895419128113No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15180.20879037748695062No Hit
CATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCT14920.20521425771444685No Hit
GACTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGG14820.2038388270327146No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14660.20163813794194305No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14500.1994374488511715No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14060.19338555385154976No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13960.19201012316981755No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13820.19008452021539243No Hit
GGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGG13670.1880213741927941No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13070.17976879010240082No Hit
TCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAGCA12790.1759175841935506No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12750.17536741192085772No Hit
ATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTT12620.17357935203460584No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA12550.17261655055739328No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12500.1719288352165272No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12400.17055340453479498No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT12210.16794008623950374No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11980.16477659567151964No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11810.16243836351257487No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11430.15721172692199245No Hit
GCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGG11350.15611138237660666No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11270.1550110378312209No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11200.15404823635400836No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11200.15404823635400836No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11050.15198509033141003No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10960.15074720271785103No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC10950.15060965964967782No Hit
CTCCAGCGATGGTCAAAGTGACTGTTGTTCCTGAACTGGAGCCAGAGAATCGCCCAGGGATCCCTTGAGGCCTCT10880.14964685817246526No Hit
GTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACA10520.14469530771822925No Hit
CCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCT10470.14400759237736316No Hit
CACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGACCATCG10290.14153181715024515No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAG10280.14139427408207195No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10210.1404314726048594No Hit
CACCAATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGA10130.13933112805947362No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10030.1379556973777414No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9940.1367178097641824No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9930.1365802666960092No Hit
CTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATA9850.13547992215062343No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9820.13506729294610376No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT9780.13451712067341087No Hit
GCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTT9780.13451712067341087No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9730.13382940533254475No Hit
GCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA9500.13066591476456066No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT9430.1297031132873481No Hit
GGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCT9390.12915294101465521No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9230.12695225192388368No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9200.126539622719364No Hit
GTTCAGGAACAACAGTCACTTTGACCATCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAG9030.12420139056041922No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8860.12186315840147445No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8750.12035018465156903No Hit
CAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGT8630.11869966783349037No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8620.11856212476531713No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8460.1163614356745456No Hit
CCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG8460.1163614356745456No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8260.11361057431108115No Hit
GCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT8210.11292285897021505No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC8210.11292285897021505No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8180.11251022976569537No Hit
CTCCACTGCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC8180.11251022976569537No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA8170.11237268669752216No Hit
GGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTTGGCAAAGCATCTCCAGAGCAGGTGATCCTGGC8120.11168497135665605No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT7950.10934673919771128No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7920.10893410999319163No Hit
AGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTT7880.10838393772049873No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT7770.10687096397059329No Hit
GGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCC7750.10659587783424684No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7690.10577061942520752No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7640.1050829040843414No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7630.10494536101616819No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC7580.10425764567530207No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTAT7570.10412010260712887No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7560.10398255953895563No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7460.10260712885722342No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7450.10246958578905019No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTC7430.10219449965270375No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7400.10178187044818408No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7350.10109415510731798No Hit
CAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGT7320.10068152590279832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC501.1114025E-955.223553
TACGACG200.006936740652.5272261
AATCGGA200.00706784252.27912556
GGTATCA3900.047.79291
GTATCAA12200.044.7024651
CTTATAC503.8277904E-641.4205131
GTGGTAT1100.040.792931
TTATACA602.898887E-740.2671742
TATCAAC13600.040.0979842
CCTATAC350.001344668739.4453963
TGGTATC1150.039.016642
ATCAACG14600.037.1151433
TCAACGC14750.036.73774
CAACGCA15350.035.30175
AACGCAG15400.034.9629676
AGAGTAC15950.033.752711
CAGAGTA16100.033.43823610
CGCAGAG16500.032.627618
GCAGAGT17050.031.7775129
CGTAAAC553.121405E-431.58996650
Produced by FastQC (version 0.11.5)