FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86184
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280.26455026455026454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCG200.006582028753.18757665
TCGAGAC200.006676137452.99716663
ATGGTAT200.006707728452.93462
GGTCTAG200.00699707852.372250
AGGTCTA200.00702979252.31051349
GTTAATT200.007414235351.61141629
GTATCAA3450.041.8875271
GGTATCA1550.039.957231
ACGGGGG801.6552804E-938.68608516
ATCAACG3750.038.491793
TATCAAC3900.037.9145662
TACGGGG1550.037.7154715
TCAACGC4000.036.945254
CAACGCA3950.036.5428435
AACGCAG4050.035.640556
ACGCAGA4100.035.2059067
AGAGTAC4200.035.1859511
CAGAGTA4250.034.77210
CCAGTAC400.002617255334.4076119
GCAGAGT4400.033.586599
Produced by FastQC (version 0.11.5)