FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133016
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGCCTGCATATGGGCAGCAGTAATAAT45990.40590777182316934No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGCCTGCATATGGGCAG43140.3807536698510877No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41590.3670733687785521No Hit
GATTATTACTGCTGCCCATATGCAGGCAGCTTCACTTTTGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT34780.30696830406631503No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG33300.2939058230422165No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32370.28569764239869516No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32050.28287332217726846No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG31760.2803137819766005No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGAACTGATGTT31330.2765186016790584No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30920.2728999413953554No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG30460.2688399810770545No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29900.2638974206895578No Hit
CTCCTGCACTGGAACCAGAACTGATGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAACAGCCAGGCAAAGC28870.25480663997684055No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27390.24174415895274207No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCTGCCCATAT27120.2393611387659133No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26650.2352129184406928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26260.231770778170829No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC26070.23009383803935687No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC24590.2170313570152584No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG23620.2084701363440587No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23590.20820535632329992No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22480.19840849555522608No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22340.19717285545835186No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21580.19046509493246344No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC20480.18075649417130915No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20320.1793443340605958No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTGTTGTTGGTACCAGGAGA20140.17775565393604326No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGC19630.17325439358314446No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGAACTGATGTTGGTGGTTATA19500.17210701349318985No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19260.16998877332711984No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19130.16884139323716524No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18900.16681141307801478No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18860.16645837305033645No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18720.16522273295346226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18620.1643401328842664No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18520.16345753281507058No Hit
GTATGTCTCCTGGTACCAACAACAGCCAGGCAAAGCCCCCAAACTCATGA18500.1632810128012314No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGAA18160.16028017256596552No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18060.1593975724967697No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17530.1547197921300317No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17460.1541019720815946No Hit
GCCTGGTCCCTCCGCCGAACACAAAAGTGAAGCTGCCTGCATATGGGCAGCAGTAATAATCAGCCTCATCGTCAG17450.15401371207467504No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT17030.15030679178405248No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT16570.1462468314657516No Hit
GTAATATATAGCCGTGTCTGCGGCGGTCATAGACCTCAGCTTCAGGGAGA16520.14580553143115366No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15890.14024515099521984No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15840.13980385096062192No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15390.13583215064924062No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15290.13494955058004476No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15120.13344913046241183No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA15030.13265479040013559No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG14860.13115437028250262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14820.1308013302548243No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT14820.1308013302548243No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC14590.12877135009567386No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14580.12868309008875425No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14380.12691788995036257No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14350.12665310992960382No Hit
GCCCATATGCAGGCAGCTTCACTTTTGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTG14250.12577050986040797No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT13820.12197532956286583No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC13720.12109272949366999No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13670.12065142945907206No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13090.11553234905773617No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13010.1148262690023795No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG13010.1148262690023795No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT12980.11456148898162073No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.1142084489539424No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACAAAAGTGAAG12890.11376714891934447No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12720.11226672880171153No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAACAGCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAAG12680.1119136887740332No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC12650.11164890875327445No Hit
GACCATCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCTGCC12550.1107663086840786No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12490.11023674864256108No Hit
GCCTGCATATGGGCAGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCC12310.10864806851800858No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12210.10776546844881274No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC12180.10750068842805398No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCC12120.10697112838653647No Hit
TATGCAGGCAGCTTCACTTTTGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11940.10538244826198394No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11910.10511766824122519No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC11890.10494114822738602No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11810.10423506817202935No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11720.10344072810975309No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCT11660.10291116806823558No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG11630.10264638804747682No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11610.10246986803363765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11350.10017510785372845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA200.006634290353.12460365
TGGTATC2750.045.06252
GTATCAA21700.044.6322631
GTGGTAT2800.044.3145981
GGTATCA7550.042.4559251
TATCAAC25950.037.0094222
ATCAACG27050.035.724213
TCAACGC27500.035.2600144
CAACGCA27750.034.8153725
AACGCAG28000.034.6273166
CATTGCG400.002639265334.38476618
CGCAGAG30150.032.5001458
CAACTCG800.003597331532.27649370
AGAGTAC30850.031.65125311
CAGAGTA31100.031.50737410
ACGCAGA31300.031.1962037
GCAGAGT31650.030.8512219
TACGGGC2750.026.25629615
AGTACGG30550.025.88477113
GAGTACG30800.025.78629712
Produced by FastQC (version 0.11.5)