FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332254
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14730.44333552041510416No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14320.43099556363505026No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13750.4138400139652194No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11910.3584606957327827No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAAT11760.3539460773986167No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10760.32384862183750984No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG10040.3021784538335129No Hit
GATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT9970.3000716319442354No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG9270.27900341305146065No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9190.27659561660657206No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT8940.26907125271629534No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8710.2621488379372408No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8440.25402252493574196No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG7840.2359640515990778No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7720.232352356931745No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG7690.23144943326491177No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGC7490.2254299421526904No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG7390.22242019659657972No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAG7350.22121629837413545No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG7210.21700265459558046No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTC7160.21549778181752513No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7110.2139929090394698No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6630.19954613037013852No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT6530.19653638481402785No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACA6480.19503151203597247No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC5930.1784779114773637No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT5880.17697303869930836No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG5840.1757691404768641No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC5820.17516719136564193No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC5320.16011846358508852No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT5220.15710871802897783No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC5140.1547009215840893No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5120.15409897247286714No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5080.15289507425042287No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG4940.1486814304718679No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG4930.14838045591625684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4870.14657460858259044No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC4740.14266193935964655No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG4680.14085609202598012No Hit
GTGTTAATTCCTGCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC4660.140254142914758No Hit
CCCCTGAGGGCCGCTTACTGACGTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA4610.13874927013670266No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA4560.1372443973586473No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4510.13573952458059196No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC4430.1333317281357034No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA4400.1324288044688702No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC4250.12791418613470418No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4250.12791418613470418No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTAT4250.12791418613470418No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACA4200.12640931335664884No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC4190.12610833880103775No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCC4170.12550638968981562No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC4020.1209917713556496No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCG3950.11888494946637211No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC3920.1179820257995389No Hit
TTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC3910.11768105124392784No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCA3890.11707910213270571No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3810.11467130568781717No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTC3750.11286545835415074No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG3580.10774889090876257No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA3570.10744791635315151No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA3530.10624401813070722No Hit
GGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGG3520.10594304357509617No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG3480.10473914535265189No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCT3410.1026323234633744No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC3370.10142842524093014No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAA3350.10082647612970799No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC3340.10052550157409693No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC3330.10022452701848586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)