FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603058
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29320.486188724799273No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27910.46280788912509246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18760.3110811895373248No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17260.2862079600967071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16840.2792434558533342No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAT16540.27426880996521064No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16540.27426880996521064No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT15990.2651486258369842No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14340.23778807345230477No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13490.22369324343595476No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCT13470.2233616003767465No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13340.22120592049189297No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12900.2139097731893118No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTA12580.20860348424198003No Hit
GAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATC12420.20595033976831414No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12050.1998149431729618No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11910.19749344175850414No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11600.1923529743407765No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11560.19168968822236004No Hit
GTTCTGACGGTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGT11310.1875441499822571No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGG10720.17776067973561416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10560.17510753526194825No Hit
GTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10550.17494171373234416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10520.1744442491435318No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9820.16283674207124355No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9780.1621734559528271No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCAGTT9780.1621734559528271No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9500.1575304531239118No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9250.15338491488380884No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8940.1482444474660812No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8810.14608876758122766No Hit
GTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATG8770.1454254814628112No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8590.1424406939299371No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8570.14210905087072886No Hit
ACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT8530.14144576475231238No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8520.14127994322270826No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8430.1397875494562712No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8180.13564201121616826No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8030.1331546882721065No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7920.1313306514464612No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7840.13000407920962825No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7540.12502943332150473No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7530.12486361179190061No Hit
GTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCC7470.12386868261427592No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA7410.12287375343665119No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7260.12038643049258944No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7150.11856239366694414No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGA7060.11706999990050708No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7060.11706999990050708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7040.11673835684129885No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7020.11640671378209061No Hit
CTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA6970.11557760613407002No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6950.11524596307486178No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGG6930.11491432001565355No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT6910.11458267695644532No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGG6700.11110042483475885No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6630.10993967412753002No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC6560.10877892342030121No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC6400.10612577894663532No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC6330.1049650282394065No Hit
CGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6280.1041359205913859No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6050.1003220254104912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA200.007382286751.7057239
GTATCAA14400.044.535451
GGTATCA4850.041.94361
TATCAAC16900.037.335312
ATCAACG18000.035.053713
CAACGCA18250.034.573525
TGTTAGA3750.033.89369670
AACGCAG18500.033.7335666
TACGGGT958.8475645E-932.65895515
ACGCAGA19550.031.9164967
TCAACGC20150.031.313494
CGCAGAG20100.031.043168
AGAGTAC20150.030.62142211
GCAGAGT21550.029.1119619
AGTACGG17650.028.90682413
GAGTACG17700.028.62802712
CGCGGTA854.2503307E-628.55793843
TTAACGG854.364805E-628.46060835
CAGAGTA22150.028.0121310
TATATAG500.007754990427.5832482
Produced by FastQC (version 0.11.5)