FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603058
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28590.47408375313817247No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24910.4130614302438571No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16950.28106749267897946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16320.27062073631392003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15870.2631587674817348No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAT15390.25519933406073714No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15190.2518829034686548No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT14910.24723990063973944No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCT13840.22949699697209888No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCATCTACTTAGCCTGGTA12630.20943259189000063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12620.20926677036039654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12440.20628198282752241No Hit
GAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATC12390.2054528751795018No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12000.1989858355249412No Hit
GTTCTGACGGTGCTGACAGTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGT11920.19765926328810826No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11890.19716179869929593No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11600.1923529743407765No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11440.18969982986711062No Hit
GTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11410.18920236527829826No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11310.1875441499822571No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGG11080.1837302548013624No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10410.17262021231788652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10340.17145946161065767No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10020.1661531726633259No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9400.1558722378278706No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9300.15421402253182945No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9220.1528874502949965No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCAGTT9150.15172669958776766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9110.1510634134693512No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9050.1500684842917265No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9020.14957101970291414No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8950.14841026899568532No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC8940.1482444474660812No Hit
GTCAGAGTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATG8760.1452596599332071No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8500.14094830016350002No Hit
ACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT8450.14011919251547944No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8010.13282304521289826No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7870.13050154379844062No Hit
GTGTTACCATCTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCC7770.12884332850239943No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA7530.12486361179190061No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7470.12386868261427592No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC7440.12337121802546355No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7390.12254211037744296No Hit
GCCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCACCGTCAGAACTGGCCGTGGACGTTCGGCCAAGGGA7210.11955732284456885No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7160.11872821519654825No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7140.11839657213734002No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7120.11806492907813178No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA6960.11541178460446591No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6640.11010549565713414No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6550.10861310189069708No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6480.10745235118346824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6480.10745235118346824No Hit
CTACTTAGCCTGGTACCAACAGAAACCTGACCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA6440.10678906506505177No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6390.10595995741703121No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGG6380.10579413588742707No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT6240.10347263447296944No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC6220.10314099141376121No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6110.1013169545881159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGG200.007368602351.72997332
CGACGTA200.007429373551.62263524
TACCGAC200.00744401451.59693521
CATACCG200.007451342451.584119
GTATCAA16200.044.902861
GGTATCA5500.040.1165731
TATCAAC18600.039.4402922
ATCAACG19750.037.1006243
CAACGCA20000.036.6125645
AACGCAG20500.035.7195786
ACGCAGA20950.035.1164257
AGAGTAC21350.034.7805511
GCAGAGT22000.033.752949
CGCAGAG21950.033.6732068
CAGAGTA22800.032.56862610
TGTTAGA3650.032.41543270
TCAACGC22700.032.2577674
GTGGTAT3500.031.5201641
TAACGGC1001.4390025E-831.06899536
AGTACGG19500.029.9705613
Produced by FastQC (version 0.11.5)