FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740250
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13420.18129010469436No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11980.1618372171563661No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11360.15346166835528538No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11260.1521107733873691No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC11230.15170550489699428No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG10280.13887200270178993No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9840.13292806484295844No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9150.12360688956433638No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC8430.11388044579533942No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8150.11009793988517394No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC8080.10915231340763257No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG8050.10874704491725769No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC7770.10496453900709221No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7700.10401891252955082No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT7670.10361364403917595No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG7420.10023640661938535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.044.24661
GTATCAA16750.040.5422331
TCGTATG501.8192887E-434.63261840
GTGGTAT2300.034.4711881
TATCAAC20400.033.1149022
ATCAACG20450.033.033943
TCAACGC20650.032.8809054
CAACGCA21300.031.8774975
AACGCAG21350.031.8028436
ACGCAGA23250.029.2039017
CGCAGAG23600.028.7707928
AGAGTAC24150.028.11555511
TGGTATC2600.027.8383542
CAGAGTA24450.027.7705810
GCAGAGT25400.026.867619
AGTACGG20350.025.74578313
GAGTACG21100.024.83064712
CGGTACG851.4448474E-424.34740629
GCTTAGA851.4508232E-424.3326031
TACGGGT2300.022.47969215
Produced by FastQC (version 0.11.5)