FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740250
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC13190.17818304626815265No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11930.16116176967240797No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT11470.15494765281999326No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11160.1507598784194529No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10900.14724755150287067No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10510.1419790611279973No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10120.13671057075312396No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC9450.1276595744680851No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9330.1260385005065856No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG9180.12401215805471125No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8090.10928740290442418No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC8000.10807159743329957No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG7850.1060452549814252No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG7810.1055048969942587No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT7720.10428909152313406No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC7570.10226274907125972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTA305.953668E-446.5456954
GGTATCA7850.046.1689221
GTATCAA20100.041.3857731
GTGGTAT2950.040.952091
TATCAAC23950.034.683612
TGGTATC3400.034.467742
TCAACGC24200.034.143624
CAACGCA24650.033.7927975
ATCAACG24550.033.663673
AACGCAG25100.033.598366
ACGCAGA26650.031.7733947
CAGAGTA27850.030.4043410
AGAGTAC27750.030.2658311
CGCAGAG28350.029.868118
TGAACGA850.005650274529.42868470
GCAGAGT28950.029.3679819
TTAACGG1601.8189894E-1228.04867235
TACGGGC5100.027.67379415
AGTACGG25850.026.6314713
ATTAGAG1950.026.4885623
Produced by FastQC (version 0.11.5)