FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917080
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTC80870.8818205609107167No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG57680.6289527631177214No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41690.454595018973263No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG38930.42449949840799056No Hit
CAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCA38020.41457669996074503No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAA34850.38001046800715316No Hit
CTATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33960.3703057530422646No Hit
GTCTCCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCATCACTT32430.3536223666419505No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT30440.33192306014742445No Hit
GTAATATACAGCCGTGTCCTCACCTCGCAGGCTGTTCATTTGCAGATACA28420.3098966284293628No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC27850.303681249182187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27810.3032450822174728No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27200.2965935360055829No Hit
GAGTAAATTCTGTCCCAGATGTACTTCCACTGAACCTTGATGGGACCCCT26500.2889606141230863No Hit
GTATATTACTGTGCGAGACCTCGCCTATCTGACTACTACTACTTCGGTAT25770.2810005670170541No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT24730.26966022593448774No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23960.26126401186374143No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23800.2595193440048851No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23060.25145025515767433No Hit
GTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCA22240.24250883238103546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21840.23814716273389452No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT21820.2379290792515375No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC21420.23356740960439656No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21280.23204082522789723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19390.21143193614515635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19120.20848780913333623No Hit
ATATTACTGTCAGCACTATGATAGTATCCCGTACTCTTTTGGCCAGGGGA19110.20837876739215772No Hit
AGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAA18970.2068521830156584No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18780.20478038993326647No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18740.20434422296855237No Hit
GAATTTACTCTAACCATCAGCAGCCTGCAGCCCGAAGATATTGCAACATATTACTGTCAGCACTATGATAGTATC18310.19965542809787587No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT18190.19834692720373362No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG18130.19769267675666244No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGC17760.1936581323330571No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17710.19311292362716448No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17290.1885331704976665No Hit
ATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGC16970.18504383477995376No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT16480.17970078946220613No Hit
GTATCAACGCAGAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTG16470.1795917477210276No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCAGCATCTGTAGGAGA15910.1734854102150303No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15730.1715226588738169No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15460.16857853186199676No Hit
GGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATT15400.16792428141492563No Hit
ATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCACTGAACCTTGATGGGAC15270.16650673877960484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15090.1645439874383914No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15050.1641078204736773No Hit
GGCCAAAAGAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCA14990.16345357002660618No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14430.1573472325206089No Hit
ACCTTGATGGGACCCCTCTTTCCAAATTCGATACATCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG14120.15396693854407467No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14060.15331268809700352No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13810.15058664456754045No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13660.1489510184498626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.14873293496750556No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13460.14677018362629213No Hit
ATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA13090.14273563920268678No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12950.14120905482618745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12710.1385920530379029No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12630.13771971910847475No Hit
GGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12590.13728355214376065No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12470.13597505124961837No Hit
ACCCAGTCTCCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCAT12440.1356479260260828No Hit
GGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC12320.1343394251319405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12020.13106817289658482No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11970.1305229641906922No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11920.1299777554847996No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATACTTGCTA11830.12899637981419287No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11810.12877829633183582No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11720.1277969206612291No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11610.12659746150826537No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11470.12507087713176604No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA11370.1239804597199808No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTC11330.12354429275526671No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11330.12354429275526671No Hit
GTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGG11250.12267195882583853No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTTT11110.1211453744493392No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11070.1207092074846251No Hit
CCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA10980.11972783181401842No Hit
GACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTC10810.11787412221398351No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10780.11754699699044793No Hit
GCACTGAACACAGAGGACTCACCATGGACTTTGGGCTGAACTGGGTTTTC10670.11634753783748418No Hit
ATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATT10620.11580232913159158No Hit
TCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10480.11427574475509224No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10460.1140576612727352No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGTATCGAATTTGGAAAGAGGGGTCCCATCAAGGTT10390.11329436908448555No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10300.11231299341387883No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTC10270.11198586819034326No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10250.1117677847079862No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10220.11144065948445064No Hit
GAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTC10140.11056832555502245No Hit
GATCTACGATGTATCGAATTTGGAAAGAGGGGTCCCATCAAGGTTCAGTGGAAGTACATCTGGGACAGAATTTA10140.11056832555502245No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG10040.10947790814323723No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9870.10762419854320235No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9870.10762419854320235No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9830.10718803157848825No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9800.10686090635495268No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA9740.10620665590788153No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9710.10587953068434597No Hit
ATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9700.10577048894316744No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9530.10391677934313255No Hit
CACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC9440.10293540367252584No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9400.10249923670781175No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9390.10239019496663324No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCCCAACCAGGACACAGCATGGAC9350.10195402800191915No Hit
GTCCCAGATGTACTTCCACTGAACCTTGATGGGACCCCTCTTTCCAAATT9250.10086361059013389No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9210.1004274436254198No Hit
GTGGAAGTACATCTGGGACAGAATTTACTCTAACCATCAGCAGCCTGCAGCCCGAAGATATTGCAACATATTA9210.1004274436254198No Hit
GTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGG9190.10020936014306277No Hit
ACATATTACTGTCAGCACTATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9180.10010031840188424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)