FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917080
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTC77990.8504165394513019No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG55040.6001657434465913No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40560.4422733022200898No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36660.39974702316046584No Hit
CAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCA36570.3987656474898591No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAA33430.36452654075980284No Hit
CTATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33330.3634361233480176No Hit
GTCTCCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCATCACTT30360.33105072621799625No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT29920.32625288960614124No Hit
GTAATATACAGCCGTGTCCTCACCTCGCAGGCTGTTCATTTGCAGATACA28540.311205129323505No Hit
GAGTAAATTCTGTCCCAGATGTACTTCCACTGAACCTTGATGGGACCCCT26790.29212282461726347No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC25130.27402189558162865No Hit
GTATATTACTGTGCGAGACCTCGCCTATCTGACTACTACTACTTCGGTAT24930.2718410607580582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24320.2651895145461683No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24280.2647533475814542No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24190.2637719719108475No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT23090.25177738038120995No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22910.24981462903999652No Hit
GTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCA22520.2455620011340341No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT21560.23509399398089587No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21230.23149561652200465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21090.22996903214550532No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20990.22887861473372006No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC20270.22102760936886642No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20210.22037335892179524No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT19520.2128494787804772No Hit
GAATTTACTCTAACCATCAGCAGCCTGCAGCCCGAAGATATTGCAACATATTACTGTCAGCACTATGATAGTATC19320.21066864395690668No Hit
AGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAA18810.205107515156802No Hit
ATATTACTGTCAGCACTATGATAGTATCCCGTACTCTTTTGGCCAGGGGA18640.20325380555676711No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAG18090.19725650979194836No Hit
GTATCAACGCAGAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTG17290.1885331704976665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17260.18820604527413093No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGC17110.1865704191564531No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16770.1828629999563833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16130.1758843285209578No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16040.17490295285035112No Hit
ATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGC15900.1733763684738518No Hit
GGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATT15680.1709774501679243No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15570.16977799101496055No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT15380.1677061979325686No Hit
ATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATTCTGTCCCAGATGTACTTCCACTGAACCTTGATGGGAC15230.16607057181489074No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15150.16519823788546256No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14670.15996423430889345No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCAGCATCTGTAGGAGA14640.1596371090853579No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14620.15941902560300084No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14510.15821956645003707No Hit
GGCCAAAAGAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCA14350.1564748985911807No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14080.1535307715793606No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13980.15244035416757534No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13860.15113185327343306No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12890.14055480437911633No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12890.14055480437911633No Hit
ATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA12880.14044576263793782No Hit
ACCTTGATGGGACCCCTCTTTCCAAATTCGATACATCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG12840.1400095956732237No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12730.13881013652025995No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12560.13695642692022506No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTC12540.136738343437868No Hit
GGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12520.13652025995551095No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12420.13542984254372573No Hit
GGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC12110.13204954856719153No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12070.13161338160247743No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATACTTGCTA11760.1282330876259432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11660.12714267021415798No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11510.12550704409648014No Hit
ACCCAGTCTCCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCAT11500.1253980023553016No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11480.12517991887294455No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11470.12507087713176604No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA11400.12430758494351639No Hit
GATCTACGATGTATCGAATTTGGAAAGAGGGGTCCCATCAAGGTTCAGTGGAAGTACATCTGGGACAGAATTTA11340.12365333449644524No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11290.12310812579055264No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11230.12245387534348148No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10740.11711083002573384No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10740.11711083002573384No Hit
ATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGGTTAGAGTAAATT10700.11667466306101976No Hit
TCGTAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10680.11645657957866272No Hit
GTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTCGG10650.11612945435512714No Hit
CACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC10640.11602041261394862No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG10570.11525712042569897No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10490.11438478649627078No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTTT10470.11416670301391374No Hit
GAGTACGGGATACTATCATAGTGCTGACAGTAATATGTTGCAATATCTTC10460.1140576612727352No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10420.11362149430802111No Hit
GGGCTGGAGTGGGTGGCAGTTATATCAGATGATGGAAGTAATAAATATTA10410.11351245256684259No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10370.11307628560212848No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10370.11307628560212848No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTC10360.11296724386094999No Hit
GACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTC10270.11198586819034326No Hit
CCATCCTCCCTGTCAGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA10190.11111353426091508No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10090.11002311684912985No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10080.10991407510795133No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGTATCGAATTTGGAAAGAGGGGTCCCATCAAGGTT10060.10969599162559429No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9800.10686090635495268No Hit
CCCCTAAACTCCTGATCTACGATGTATCGAATTTGGAAAGAGGGGTCCCA9580.10446198804902518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9570.10435294630784665No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9500.10358965411959699No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9460.1031534871548829No Hit
GCACTGAACACAGAGGACTCACCATGGACTTTGGGCTGAACTGGGTTTTC9430.10282636193134732No Hit
ACATATTACTGTCAGCACTATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9330.10173594451956208No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9230.10064552710777686No Hit
GTGCTGACAGTAATATGTTGCAATATCTTCGGGCTGCAGGCTGCTGATGG9210.1004274436254198No Hit
ATGATAGTATCCCGTACTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9190.10020936014306277No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9190.10020936014306277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGG200.007133781452.1575946
GTGGTAT2250.052.1444931
ACCTATC200.00738258751.7073432
ACTAAGC200.007443377351.6002128
GTATCAA20900.046.56021
TCTGGAG10800.041.8904570
TATCAAC24400.040.109492
AACGAAT350.001365916639.3208817
CAAAACG350.001367015439.3144514
ATCAACG25500.038.1961563
TCAACGC25750.037.6772734
CAACGCA26500.036.6069455
AACGCAG27000.036.0564466
GTATATA601.3402821E-534.5073851
GAATTAG501.8900666E-434.40952320
ACGAATT400.002631113934.4057718
CGCAGAG28450.034.2187738
CAGAGTA29000.033.4511710
GCAGAGT29300.033.2260789
ACGCAGA29800.032.7840277
Produced by FastQC (version 0.11.5)