FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402992
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18160.4506292928891889No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16590.411670703140509No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15200.3771787032993211No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14360.3563346170643586No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13560.3364831063643943No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13380.3320165164569024No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13290.32978322150315637No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12920.3206018978044229No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12590.31241314964068767No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12450.3089391352681939No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11460.28437289077698813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11000.2729582721245087No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA10180.2526104736570453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9740.24169214277206497No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGAC9530.23648112121332435No Hit
GATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTA9480.2352404017945766No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9320.23127009965458373No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9290.23052566800333507No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9270.23002938023583597No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8800.21836661769960697No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT8590.2131555961408663No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCA8560.21241116448961767No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG8250.20471870409338153No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTC7890.19578552427839757No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7690.1908226466034065No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7540.18710048834716322No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT7330.1818894667884226No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7330.1818894667884226No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGT7170.17791916464842975No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA7140.1771747329971811No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTTCTGTGCAG7050.1749414380434351No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC6940.17221185532219002No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG6790.16848969706594671No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG6760.16774526541469806No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC6700.16625640211220075No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG6590.16352681939095565No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA6580.1632786755072061No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG6480.16079723666971057No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6380.15831579783221503No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAGCTCCCAGGGGCGGCCCC6200.15384920792472306No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG6200.15384920792472306No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC6170.1531047762734744No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTC6170.1531047762734744No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTA6130.1521122007384762No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6100.15136776908722754No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6060.15037519355222934No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC5980.1483900424822329No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5920.14690117917973558No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5840.14491602810973916No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCT5750.14268273315599317No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG5620.139456862667249No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG5590.1387124310160003No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC5540.13747171159725255No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC5470.13573470441100569No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5420.1344939849922579No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5340.13250883392226148No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG5220.12953110731726683No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5140.12754595624727041No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT5020.12456822964227578No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC4960.12307936633977846No Hit
GTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTT4940.12258307857227935No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG4920.12208679080478024No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC4900.12159050303728114No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC4840.12010163973478381No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC4820.11960535196728472No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4800.1191090641997856No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC4770.11836463254853694No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4750.11786834478103785No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG4740.1176202008972883No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG4740.1176202008972883No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4720.11712391312978918No Hit
GTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAG4710.11687576924603962No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGG4700.11662762536229006No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG4680.11613133759479097No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTC4640.11513876205979275No Hit
GGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC4610.1143943304085441No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAAC4570.11340175487354587No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG4540.11265732322229723No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG4500.111664747687299No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGG4450.11042402826855124No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG4340.10769444554730614No Hit
GGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG4290.10645372612855838No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG4270.10595743836105927No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4250.10546115059356018No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA4210.10446857505856195No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC4190.10397228729106284No Hit
CCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA4180.10372414340731331No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT4180.10372414340731331No Hit
CTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG4140.10273156787231508No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG4120.10223528010481597No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG4100.10173899233731688No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC4070.10099456068606821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4300.045.7475621
GTGGTAT1500.043.714341
GTATCAA11800.041.823041
CCTACAC350.001358641939.3582763
TATCAAC12700.038.8061032
TGGTATC1700.036.4914632
ATCAACG13500.036.4792943
TCAACGC13800.035.4279174
CAACGCA14050.034.793215
AACGCAG14250.034.5464676
TGCTAGC400.002607595434.464162
GCTTGTC851.2016426E-732.4812431
ACGCAGA15450.031.6404277
GACAGGT553.2470372E-431.3739281
CGCAGAG15500.031.3162578
AGAGTAC16100.030.14919311
CAGAGTA16500.029.62694410
GCAGAGT16400.029.5976839
TAGCCTG605.484814E-428.6880635
TACACCC1103.7280188E-828.166465
Produced by FastQC (version 0.11.5)