FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047769
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAAT53410.5097497635452088No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51660.493047608776362No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC48870.46641960203060023No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC47360.4520080284871952No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46950.44809495222706536No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42620.4067690492847183No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT42100.4018061232962609No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40110.38281338730197206No Hit
GATTATTACTGCTGCTCCTATGCAGGAATTAACACTTATGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT38250.3650613828047976No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG37670.35952581150997975No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTC37340.35637626232499725No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG36970.352844949602441No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36320.3466412921168693No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34710.33127530972953007No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGC33370.318486231220813No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAG32920.3141913914231095No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAATTATAATATTGTCTCCTGGTACCAACAACA30840.29433968746927996No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG30510.2911901382842974No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30440.2905220520935435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30380.28994940678718306No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28830.2751560697062043No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28140.26857064868305897No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27930.26656639011079736No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC27110.2587402375905376No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25590.2442332231627391No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25200.2405110286713961No Hit
TTATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC23150.22094564737074682No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22900.21855962526091152No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGC22730.21693713022622355No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC22480.21455110811638825No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21490.20510246056144057No Hit
GTGTTAATTCCTGCATAGGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC20950.19994865280419635No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20950.19994865280419635No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC20870.19918512572904903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19900.18992735994288817No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC19380.1849644339544308No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19380.1849644339544308No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCC19220.1834373798041362No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18620.17771092674053157No Hit
CCCCTGAGGGCCGCTTACTGACGTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA18460.17618387259023696No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18410.1757066681682699No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17870.17055286041102571No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17460.16663978415089584No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17220.164349202925454No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17160.16377655761909352No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16960.1618677399312253No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG16860.1609133310872912No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG16850.16081789020289777No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACT16360.15614128686762063No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16100.15365982387339194No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15930.15203732883870394No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15660.14946042496008186No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTAT15550.14841057523175433No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15510.1480288116941807No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC15370.14669263931267293No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15310.14611999400631245No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14930.14249324039936284No Hit
ATATTATAATTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGAT14880.1420160359773958No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14790.14115706801785508No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14770.14096618624906826No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTC14750.14077530448028144No Hit
GGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAATTAACA14580.13915280944559347No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT14180.135335174069857No Hit
TCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACC14160.13514429230107017No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCA14090.1344762061103163No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14060.13418988345713606No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13980.13342635638198877No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCT13890.13256738842244808No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGC13800.13170842046290737No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCG13640.1301813663126128No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA13420.12808166685595773No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG13320.12712725801202365No Hit
GTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAAC13290.1268409353588434No Hit
ATAATATTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGC13130.12531388120854883No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCC13020.1242640314802213No Hit
GGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGG12740.1215916867172058No Hit
GTATCAACGCAGAGTACGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG12500.11930110549176394No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCCTATGCAGGAA12470.1190147828385837No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGATTGGGCC12450.11882390106979687No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12390.1182512557634364No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12220.11662876072874842No Hit
CACCCAGGCAAAGCCCCCAAACTCATGATTTTTGACGTCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCT12130.11576979276920771No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12060.11510170657845385No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12020.11471994304088019No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11990.11443362038769997No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11940.1139564159657329No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11780.11242936181543833No Hit
GCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAG11630.11099774854953716No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11610.11080686678075033No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC11590.11061598501196351No Hit
CTCAGGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCC11430.10908893086166893No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTT11360.10842084467091505No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11340.10822996290212825No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG11340.10822996290212825No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11310.10794364024894801No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG11290.10775275848016119No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11180.10670290875183365No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAATATTATAA11170.10660746786744024No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11140.10632114521426002No Hit
TTGTCAGGGAGGCCGTGTTGCCAGATTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGACGT11020.10517585460153908No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAATCTGGCAACACGG10900.10403056398881816No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATCTGCC10850.1035533595668511No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10810.10317159602927745No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA10780.10288527337609721No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG10770.10278983249170381No Hit
GTTCCGAAGACATAAGTGTTAATTCCTGCATAGGAGCAGCAGTAATAATC10620.10135821922580264No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10600.10116733745701581No Hit
AATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG10540.10059469215065535No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA10520.10040381038186853No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10490.1001174877286883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)