FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364868
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG200.00743369551.6109426
TTATCCG200.00743369551.610944
CGAATTA405.0752322E-543.00911315
TGGTATC2150.036.80782
GTATCAA10350.036.5731471
GGTATCA5050.034.752721
TAACGGC501.8538401E-434.51608336
TTAACGG501.858339E-434.50185435
CTATACA400.002628476634.407294
TATTAGA709.918585E-734.407292
GTGGTAT2350.032.2154961
TATCAAC11900.032.0941962
ATTAGAG652.3602266E-531.7605763
TCAACGC11900.030.9376494
ATCAACG12100.030.7106383
CAACGCA12250.030.0537155
AACGGCC605.395913E-428.76735937
TTTAACG605.422022E-428.74364934
GTGTAGA605.4965634E-428.6766741
AACGCAG12900.028.539386
Produced by FastQC (version 0.11.5)