FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364868
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.11428790685946699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG252.616606E-454.97722612
ATATCCG200.00724535951.94692641
GTATCAA12400.038.3347661
TATCAAC14400.032.9833072
GGTATCA6000.032.7234461
ATCAACG14600.032.2647483
GTCTTAC751.6856775E-632.1493531
GTGGTAT3000.032.1493531
AACGCAG14700.032.0232966
TCAACGC14850.031.699834
CAACGCA15200.030.9698985
CGAACGC450.0047108730.54290213
AGAGTAC15550.030.493811
ACGCAGA15550.030.272837
CGCAGAG15850.030.1334158
TGGTATC3200.030.1151922
GCAGAGT16650.028.8919339
CAGAGTA16650.028.89193310
GTGTATA605.540255E-428.63789616
TACGGGG6300.027.81585715
Produced by FastQC (version 0.11.5)