FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004325
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT40970.4079356781918204No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38040.37876185497722353No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA34890.3473975057874692No Hit
GTAATATACAGCCGTATCCTCGACTCTCAGGCTGTTCATTTGTAGATACA27930.2780972294824882No Hit
GTTATTATACTGTTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACT27750.27630498095735945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24840.24733029646777688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23010.2291091031289672No Hit
GTATATTACTGTGCGAGAGAATCCATTTCAGGAAATGGGTATGAGAAGTA22450.22353321882856644No Hit
GTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCAGTGGCCCTAGT22250.22154183157842333No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATT21950.21855475070320865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21220.2112861872401862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20530.2044159012271924No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.19724690712667714No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19540.1945585343389839No Hit
CTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTTCCAGCAGAAGCCTGACCAGGCTCCCAGGCT18450.18370547382570382No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17770.17693475717521717No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCAC17740.17663604908769573No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17400.1732506907624524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17200.17125930351230928No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17180.17106016478729494No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGCGTCACCACCAACTTAGCCTGGTT17020.16946705498718045No Hit
GAGTTCACTCTCACCATCGACAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAACAGTATAATAACTGG16540.16468772558683695No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16390.16319418514922956No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16220.16150150598660792No Hit
CATTTATGGTGCATCCACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA15830.1576183008488288No Hit
CTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15710.15642346849874295No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14740.14676524033554875No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14710.14646653224802728No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTG13940.13879969133497622No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13910.13850098324745477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13670.136111318547283No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13590.13531476364722575No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13160.131033281059418No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCTGGCTGGGATACCA12950.12894232444676773No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGTCGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12940.12884275508426057No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12630.12575610484653874No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12490.12436213377143852No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12380.1232668707838598No Hit
CCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGGC11930.11878624947103775No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11900.11848754138351629No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11800.11749184775844473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11670.1161974460458517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11610.11560002987080875No Hit
GTTTATTACTGTCAACAGTATAATAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAA11460.11410648943320141No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11380.11330993453314414No Hit
CACTAGGGCCACTGGTATCCCAGCCAGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCGACAG11290.11241381027057974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11110.11062156174545092No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10850.10803275832026486No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10820.10773405023274338No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10740.10693749533268614No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10700.10653921788265752No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10650.10604137107012171No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10570.10524481617006447No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10530.10484653872003585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10400.10355213700744281No Hit
GGATACCAGTGGCCCTAGTGGATGCACCATAAATGAGGAGCCTGGGAGCCTGGTCAGGCTTCTGCTGGAACCAGG10390.10345256764493566No Hit
CTGCTACTCTGGCTCCCAGAAACCACTGGAAGAGTAGTGATGACGCAGTC10370.10325342891992136No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAATCCCTGAATCTGAATCCAAAGCCAACACA10210.10166031911980683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)