FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911110
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26250.28811010745135057No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25290.27757350923598684No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG23390.2567198252680796No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGAATTT21340.23421979782902175No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21170.2323539418950511No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18970.20820757098484266No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18870.20711000867074228No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18620.2043661028854913No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17540.1925124298932072No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATTT16890.1853782748515547No Hit
GACGTAGGACGGTGACGTAGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTAATAAT16730.1836221751489941No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16160.17736606995862192No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATTGGATGGATCGATCCTTACAGTGGTGG16140.17714655749580183No Hit
CTCCTGCACTGGAAGCAGCAGTGAATTTTATCACAACTATGTCTCCTGGTACCAACGACACCCAGGCAAAGCCCC16100.17670753257016167No Hit
ACGTAGGACGGTGACGTAGGTCCCAGTTCCGAAGACATAAGTGCTGCTGC15500.17012215868555938No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14910.16364654103236712No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14770.16210995379262658No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14680.16112214770993624No Hit
GATTATTACTGCAGCTCATTTACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCTACGTCACCGTCCTACGT14520.1593660480073756No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13890.1524514054285432No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13870.15223189296572315No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13860.15212213673431307No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACGTAGGTCCCAGTTCCGAAGACATAAGTGCTG13550.14871969356060188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13180.14465871299843047No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12210.13401235855165677No Hit
GTAATACACGGCCGTGTCGTCAGATTTCAGTCTGCTCAACTCCATGTAGGCTGTGCTGCTGGACGTGTCCCTCGT12100.13280504000614635No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11980.1314879652292259No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11570.1269879597414143No Hit
GTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGAATTTTATCACAACT11530.12654893481577417No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11520.12643917858436413No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAATGAAGGTCTCCTGCGCGGCTTCTGGGTACACCTTCACCGA11410.12523186003885373No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11320.12424405395616336No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10990.12062209831963211No Hit
GTTTGGGGGCTTTGCCTGGGTGTCGTTGGTACCAGGAGACATAGTTGTGA10910.11974404846835178No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10850.11908551107989156No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10730.1177684363029711No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10690.11732941137733094No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10650.1168903864516908No Hit
GTGTATATAGAAGTCGGTGAAGGTGTACCCAGAAGCCGCGCAGGAGACCTTCATTGAGGCCCCAGGCTTCTTCAC10530.11557331167477035No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10370.11381721197220973No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10350.11359769950938965No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10290.1129391621209294No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10080.1106342812613186No Hit
GTGACGTAGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTAATAATCAGCCTCGTC10050.1103050125670885No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9860.10821964417029777No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9840.1080001317074777No Hit
GCCTTGGGCTGACGTAGGACGGTGACGTAGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTG9580.1051464696908167No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9550.10481720099658658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.040.4144971
GTATCAA13550.038.631221
TATCAAC15500.033.5453072
CGTATGC1154.5474735E-1133.10576641
CTCGTAT1154.5474735E-1133.0656439
CAACGCA16200.032.0958185
ATCAACG16400.031.7044033
TCAACGC16450.031.6080364
CAACGGA553.2626963E-431.35346632
AACGCAG16700.031.1348676
GCCGTTA450.004664490530.60961215
CTTATAC1302.0190782E-1029.1395931
CGCAGAG17800.029.0157598
GTGGTAT1800.028.6980841
AGAGTAC18150.028.45778711
TACTATA854.4933586E-628.3573472
TTATACA1353.1286618E-1028.0572682
CAGAGTA18450.027.99505810
CGAGACG1601.8189894E-1227.97602723
TATAAGG500.007806780727.5471382
Produced by FastQC (version 0.11.5)