FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486720
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGA26860.5518573307034845No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23890.490836620644313No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17830.36632971729125574No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16990.34907133464825774No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15840.3254437869822485No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15720.3229783037475345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15550.3194855358316897No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15110.31044543063773833No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14810.3042817225509533No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14380.29544707429322814No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14280.29339250493096647No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14020.28805062458908615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12940.2658612754766601No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12530.2574375410913873No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12510.2570266272189349No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11910.2446992110453649No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11000.2260026298487837No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10540.21655161078238No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10530.21634615384615385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10430.21429158448389218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10410.21388067061143984No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10360.21285338593030903No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10250.2105933596318212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10040.20627876397107167No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9980.20504602235371466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9960.20463510848126232No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9440.19395134779750164No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9230.18963675213675213No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8990.18470578566732412No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGTAATACACTGCA8840.18162393162393162No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA8700.17874753451676528No Hit
GTGTTAGCAGCACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCTTCTATGATGCAT8680.17833662064431294No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC8610.17689842209072978No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8590.17648750821827744No Hit
CTTCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8540.1754602235371466No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8370.17196745562130178No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7830.16087278106508876No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7750.1592291255752794No Hit
GTCTCCAGTCACCTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAG7620.15655818540433925No Hit
CTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCACCTATTTAGCCTGGTACC7560.15532544378698224No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7470.15347633136094674No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7450.15306541748849442No Hit
TCATAGAAGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGGTGCTGCTAACACTCTGA7280.1495726495726496No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7130.14649079552925706No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7040.14464168310322156No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7030.1444362261669954No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6920.14217619986850755No Hit
GAGTGTTAGCAGCACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGG6920.14217619986850755No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6780.13929980276134124No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC6730.1382725180802104No Hit
GTCAGAGTGTTAGCAGCACCTATTTAGCCTGGTACCAGCAGAAACCTGGC6710.13786160420775806No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6640.13642340565417488No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6140.12615055884286655No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6070.12471236028928337No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC5980.12286324786324786No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5960.12245233399079553No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5920.12163050624589086No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5890.12101413543721236No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5810.11937047994740302No Hit
GAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCACCTATTTAGCCTGGTACCAGCAGAAACCTGGC5570.11443951347797501No Hit
CCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG5570.11443951347797501No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5480.11259040105193951No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5450.11197403024326101No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5440.11176857330703485No Hit
GCAGAAACCTGGCCTGGCGCCCAGGCTCCTCTTCTATGATGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT5330.10950854700854701No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5320.10930309007232085No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5200.10683760683760685No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5180.10642669296515452No Hit
CTCCAGTCACCTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCA5080.10437212360289283No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5060.10396120973044051No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5010.10293392504930968No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4940.10149572649572651No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT4930.10129026955950032No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT4900.10067389875082183No Hit
GTCTGGGATGCCAGTGGCCCTGCTGGATGCATCATAGAAGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTA4890.10046844181459567No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCAGCACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCG4880.10026298487836949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC150.002409468668.6012612
TATATCG200.00694807452.5028355
GTATCTA250.00802544250.6213869
CGTCGGA556.1785954E-446.1857670
TACGGAT1304.802132E-1043.9652970
GTATCAA10350.042.86161
GGTATCA3800.041.628741
ACGGGTA350.001384580739.20877516
TATCAAC12200.036.617572
TCAACGC12500.035.6726534
CAACGCA12550.035.2572175
ATCAACG12750.034.994763
AACGCAG12900.034.300636
ACGCAGA13550.032.655217
AGAGTAC13950.031.22709311
CGCAGAG14250.031.0510928
CGAGCCT450.00475294930.4894478
GCAGAGT15000.029.7272119
CAGTTCG8200.028.65488670
CAGAGTA15600.028.3639810
Produced by FastQC (version 0.11.5)