FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807379
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46460.5754422644136149No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA35360.4379603631008485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32750.4056335376570359No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30430.3768985817069802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29210.361787958319451No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28180.34903062873817625No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGGGG26450.3276032693443847No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26230.32487840283187946No Hit
GAGTATTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATC25100.3108824975631024No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23620.29255157738806686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23320.28883585032555964No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGAC22980.2846246929880515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21990.27236279368177774No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGCTG21710.268894781756771No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT21620.2677800636380188No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20970.2597293216692532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20440.25316487052549047No Hit
GTATTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC19400.24028368337546555No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGGGGCTACTTAGCCTGGTA18920.23433852007545405No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18910.23421466250670378No Hit
GTCTGGGACGGACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGATT18630.23074665058169705No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18170.22504920241918605No Hit
GTTTATTACTGTCAGCAGCGTAGCGACTGGTCCGCGCTCACTTTCGGCGG18050.22356291159418315No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC17440.21600759990041848No Hit
GCGTAGCGACTGGTCCGCGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT16910.2094431487566558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16780.2078330003629027No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC16540.20486041871289692No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16240.20114469165038973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16000.19817211000038396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15860.19643810403788062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15310.1896259377566174No Hit
GACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGATTTTGCAGTTTA15080.18677721367536188No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14790.18318534418160493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14670.18169905335660205No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14520.17984118982534844No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13600.16844629350032636No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13450.16658842996907275No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13390.16584528455657133No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13300.16473056643781916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12900.15977626368780956No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATCCCACCCAGGTT12830.1589092607065579No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC12810.1586615455690574No Hit
GTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCA12770.15816611529405644No Hit
CTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATCCCA12400.15358338525029758No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12380.15333567011279708No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC12250.15172552171904397No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12190.15098237630654252No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11990.14850522493153773No Hit
GTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCACTGAACCTGGGTGGGATGCCAGTGG11980.14838136736278748No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11850.14677121896903436No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11780.1459042159877827No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11660.14441792516277982No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGCTGCTGA10990.13611946805651373No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10970.13587175291901324No Hit
GTCCCAGACCCACTGCCACTGAACCTGGGTGGGATGCCAGTGGCCCTCTC10750.13314688640650796No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10630.1316605955815051No Hit
GGTTCAGTGGCAGTGGGTCTGGGACGGACTTCAGTCTCACCATCAGCAGC10570.13091745016900364No Hit
GGCCACTGGCATCCCACCCAGGTTCAGTGGCAGTGGGTCTGGGACGGACT10440.12930730177525052No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10320.12782101095024764No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10260.12707786553774622No Hit
GTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10120.12534385957524286No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10100.1250961444377424No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10040.12435299902524093No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATC9870.12224742035648686No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9840.12187584765023614No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9780.1211327022377347No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9770.12100884466898446No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9740.12063727196273373No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG9690.12001798411898253No Hit
TTATTACTGTCAGCAGCGTAGCGACTGGTCCGCGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAA9640.11939869627523134No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9610.11902712356898061No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9520.11791240545022845No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9520.11791240545022845No Hit
GTCGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGT9470.11729311760647725No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9460.11716926003772703No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9450.11704540246897678No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9400.11642611462522558No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC9340.11568296921272414No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC9330.1155591116439739No Hit
GGTCTGGGACGGACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGAT9280.11493982380022269No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9060.11221495728771741No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9050.11209109971896716No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8960.11097638160021502No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8890.11010937861896333No Hit
GAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC8880.1099855210502131No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8860.10973780591271261No Hit
CACCATCAGCAGCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCGACTGGTCCGCGCTCA8810.10911851806896143No Hit
CTATGATACATCCGAGAGGGCCACTGGCATCCCACCCAGGTTCAGTGGCAGTGGGTCTGGGACGGACTTCAGTCT8770.10862308779396046No Hit
CTGGCATCCCACCCAGGTTCAGTGGCAGTGGGTCTGGGACGGACTTCAGT8700.10775608481270879No Hit
CAGTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCA8680.1075083696752083No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCCCCTA8650.10713679696895757No Hit
GTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATCCCACC8620.10676522426270685No Hit
GGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8500.10527893343770399No Hit
GATCTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTAC8350.10342106990645038No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8200.10156320637519677No Hit
CATCTATGATACATCCGAGAGGGCCACTGGCATCCCACCCAGGTTCAGTG8120.10057234582519486No Hit
CCCAGGTTCAGTGGCAGTGGGTCTGGGACGGACTTCAGTCTCACCATCAG8080.10007691555019389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.047.6098371
TCGTATG1300.045.25319340
CGTATGC1350.043.60158541
GTATCAA17150.043.3470421
ACGAGAC1500.041.30010222
CGATTAA350.001255156440.00244557
ACCGATT350.001311341239.6477845
GTACGAC350.001364094439.33099411
CCGTCCG350.001364925439.326129
CTTAACG458.8533154E-539.14278463
TATCAAC19350.038.4186972
ATCAACG20050.037.0773963
TCAACGC20400.036.6099744
CAACGCA20500.036.431395
CTTATAC1550.035.5269661
AACGCAG21050.035.47956
CGAGACA1750.035.40447223
TAACGCT400.002304598735.34728665
AAATCGA601.7806797E-434.7586569
CGCTAAG450.003116870533.2360768
Produced by FastQC (version 0.11.5)