FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489991
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.043.740241
TATCAAC15200.041.2900242
GGTATCA5650.039.675881
TCAACGC16150.039.0747264
ATCAACG16150.038.647683
CAACGCA16300.038.5035825
AACGCAG17050.037.012136
AGAGTAC18300.034.29203811
ACGCAGA18700.033.742917
GGTCTAA700.002954013433.5950770
CAGAGTA18750.033.46903210
CGCAGAG18900.033.385848
GCAGAGT19800.031.8683039
GTGTACG450.00462832830.6555541
CGGGTAC450.004632952630.64929617
AGTACGG14650.030.36163113
GAGTACG14750.030.1557912
ACGGGTC854.4408753E-628.3956716
TACGGGT2800.028.32323515
TACGGGA5850.026.5234315
Produced by FastQC (version 0.11.5)