FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180355
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36620.310245646436877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30670.259837082911497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26550.2249323296804775No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25440.21552837917406206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24180.20485362454515801No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23560.19960096750553857No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23280.1972287998102266No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21230.1798611434695494No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG20340.17232103900945056No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19880.16842390636715227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19550.1656281372976774No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18960.16062964108255567No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18880.15995187888389512No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA18550.15715610981442024No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18420.15605474624159682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18400.15588530569193168No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18240.1545297812946105No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17730.15020904727814935No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17710.1500396067284842No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG16410.13902597100024994No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA16220.13741628577843107No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16020.13572188028177962No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15950.13512883835795164No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15900.13470523698378878No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15010.1271651325236899No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT14880.1260637689508665No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT14130.11970974833842361No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA13770.11665981844445103No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13440.11386404937497618No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13300.11267796552732016No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG13270.11242380470282244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13130.11123772085516645No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12940.1096280356333476No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT12830.1086961126101893No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA12330.10446009886856072No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC12170.1031045744712396No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA12170.1031045744712396No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG12030.10191849062358357No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT12020.101833770348751No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC11970.10141016897458816No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11820.10013936485209958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)