FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509969
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25060.4914024185783842No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24710.48453925630773637No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23810.46689112475464195No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC21420.4200255309636468No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20020.3925728818810555No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT19640.3851214485586379No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19250.3774739248856303No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18780.3682576784079032No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17970.35237436001011824No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGG16400.32158817496749803No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15820.31021493463328165No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15810.31001884428269166No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCA15800.30982275393210174No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTC15680.3074696697250225No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15370.30139086885673444No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15140.29688079079316587No Hit
GCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGT15100.2960964293908061No Hit
ATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC15000.2941355258849067No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14890.2919785320284174No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14890.2919785320284174No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGC14710.2884489057177986No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13750.2696242320611645No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC13470.2641337022446462No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGAC13160.25805490137635817No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13150.25785881102576824No Hit
GATTATTTCTGTGCAGCCTGGGATGACGGCCTGATTGGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTA12660.24825038384686127No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11760.23060225229376688No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTA11730.23001398124199707No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11550.2264843549313782No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA10820.2121697593383127No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10810.2119736689877228No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10790.21158148828654288No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10740.21060103653359322No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCA10730.21040494618300326No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10470.2053065970676649No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10430.20452223566530514No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10170.1994238865499668No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10070.1974629830440674No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG9760.19138418217577932No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9420.18471711025572143No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTC9330.182952297100412No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9280.1819718453474623No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGG9250.1813835742956925No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9190.18020703219215284No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC9180.1800109418415629No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9180.1800109418415629No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9150.17942267078979313No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGT9100.17844221903684343No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTTCTGTGCAG8870.17393214097327483No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8810.17275559886973524No Hit
GTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTT8800.17255950851914528No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8600.16863770150734653No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8540.1674611594038069No Hit
GTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAG8490.1664807076508572No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8450.16569634624849747No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8390.16451980414495782No Hit
GATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGG8320.16314717169082826No Hit
CCCCTGGGAGCTGCTGGTACCAGGTTACAGCATTAGTTGGGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA8280.16236281028846852No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8280.16236281028846852No Hit
ATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG8250.1617745392366987No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC8080.15844100327666977No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8040.15765664187431No Hit
GACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT8020.15726446117313012No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7900.15491137696605087No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAACCTGGTACCAGCAGCTCCCAGGGGCGGCCCC7890.15471528661546094No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7760.15216611205779176No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7640.1498130278507125No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG7590.14883257609776282No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7460.14628340154009362No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT7400.145106859436554No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7210.14138114277534516No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCTTC7200.14118505242475524No Hit
GGTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC7130.13981241997062566No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC6910.13549843225764704No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG6850.1343218901541074No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG6830.13392970945292754No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG6780.13294925769997784No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGG6710.1315766252458483No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6690.1311844445446684No Hit
GATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTACCAGGTTACAG6690.1311844445446684No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC6520.12785090858463946No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCT6500.12745872788345958No Hit
GGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG6450.12647827613050988No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6410.12569391472815014No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6350.12451737262461052No Hit
GTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGC6270.12294864981989102No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG6270.12294864981989102No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCCAACTAATGCTGTAAC6220.12196819806694133No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC6160.12079165596340169No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6120.12000729456104195No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG6080.1192229331586822No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA6070.11902684280809224No Hit
GGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCC6010.11785030070455263No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT5900.11569330684806332No Hit
CTCCAGCATGGCCAGCTTCCCTCTCTTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG5880.11530112614688344No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT5860.11490894544570357No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG5780.11334022264098406No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5740.11255586123862431No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC5710.1119675901868545No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5650.11079104808331486No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC5600.10981059633036518No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA5500.1078496928244658No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG5480.10745751212328591No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT5480.10745751212328591No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC5480.10745751212328591No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTA5470.10726142177269599No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT5410.10608487966915635No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA5400.10588878931856642No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG5360.10510442791620667No Hit
CGGTGACCCTGGTCCCAGTTCCGAAGACATAACCAATCAGGCCGTCATCCCAGGCTGCACAGAAATAATCAGCCT5280.10353570511148716No Hit
GTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCT5260.10314352441030729No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA5220.10235916300794755No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC5180.1015748016055878No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC5150.10098653055381798No Hit
TTATTAGTATAGATGAGGAGTTTGGGGGCCGCCCCTGGGAGCTGCTGGTA5150.10098653055381798No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAG5150.10098653055381798No Hit
CTCCTCATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT5130.1005943498526381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)