FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528077
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10450.1978878080279959No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACGGAGCTCAACCTCAGGGAGA9310.17630004715221453No Hit
GTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8550.16190820656836027No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8380.15868897906934026No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7690.1456227027497884No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7170.13577565392925653No Hit
CTATTACACTGGGAGCCCCAACTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAAGTCCAAGAG7120.13482882231189772No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6800.1287690999608012No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6420.12157317966887404No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGTAGACTGGAGCCTGAAGATT6310.1194901501106846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6110.11570282364124929No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5990.1134304277595881No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5940.11248359614222925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5910.11191549717181395No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5820.11021120026056806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5690.1077494380554351No Hit
GTACCATACAGCTGACAGTAAAACACTGCAAAATCTTCAGGCTCCAGTCTACTGATAGTGAGAGTGAAGTCTGTC5590.10585577482071742No Hit
GTCTCTGGTGCCTCCGTCAGTACTTACTACTGGAGCTGGATCCGGCAGCC5560.10528767585030213No Hit
GTTTTACTGTCAGCTGTATGGTACCTCAGCTTGGACGTTCGGCCAAGGGA5530.10471957687988684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5500.10415147790947153No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT5460.10339401261558448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG250.001850694573.31299670
GCCTAAT350.007017350852.36642570
ACCGTCG200.00736083451.74298
GTATCAA8500.043.833431
CTTATAC803.45608E-1143.123171
TATCAAC9200.039.7445452
TCAACGC9400.038.5319444
ATCAACG9500.038.4894523
CAACGCA9400.038.164975
AACGCAG9600.037.369876
GGTATCA3400.036.527861
CGCAGAG10250.035.0000728
CTAGCGC709.683081E-734.51814328
TATACAC905.185939E-934.4952663
TGTTAGA2700.033.941270
AGAGTAC10650.033.68551611
TTATACA958.789357E-932.6797262
TCGTATG751.6176236E-632.3241340
CTCGTAT652.2648228E-531.95676639
GCAGAGT11450.031.6332159
Produced by FastQC (version 0.11.5)