FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284251
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG252.578538E-455.136361
CGGTAAT200.00695948752.4753455
CGTTTAA200.00695948752.4753455
GTATCAA7500.043.190151
GGTATCA3150.040.4770931
CAACGCA8950.036.1928635
AACGCAG8950.036.1928636
TCAACGC9000.035.991794
TATCAAC9150.035.7783742
ATCAACG9250.035.019043
TACGGGC1700.032.4217415
AGAGTAC10150.031.89706411
GTGGTAT1305.456968E-1231.8094391
TGGTATC1305.456968E-1231.8094392
CAGAGTA10300.031.43254310
ACGCAGA10350.031.2861987
CGGAACA802.093575E-631.26416467
ACGGAAC703.2049935E-530.36568666
CGCAGAG10750.030.1167588
ACGGGCT953.2268144E-729.01403216
Produced by FastQC (version 0.11.5)